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Vol. 20, No. 4, 2007
Issue release date: June 2007
Section title: Original Paper
Skin Pharmacol Appl Skin Physiol 2007;20:187–194
(DOI:10.1159/000101388)

Kinetic Characteristics of Acidic and Alkaline Ceramidase in Human Epidermis

Houben E. · Uchida Y. · Nieuwenhuizen W.F. · De Paepe K. · Vanhaecke T. · Holleran W.M. · Rogiers V.
aDepartment of Toxicology, Dermato-Cosmetology and Pharmacognosy, Vrije Universiteit Brussel, Brussels, Belgium; bDepartment of Dermatology, University of California San Francisco and VA Medical Center, San Francisco, Calif., USA; cTNO Quality of Life, Zeist, The Netherlands

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 10/24/2006
Accepted: 12/22/2006
Published online: 3/29/2007

Number of Print Pages: 8
Number of Figures: 7
Number of Tables: 0

ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)

For additional information: http://www.karger.com/SPP

Abstract

It has recently become evident that at least five ceramidase (CDase) isoforms are present in human epidermis, and that specifically acidic CDase (aCDase) and alkaline CDase (alkCDase) activities increase during keratinocyte differentiation, and thus might play a pivotal role(s) in permeability barrier function. Prior to investigating their possible roles in the epidermal barrier function, it is necessary to characterize basic kinetic parameters for these enzymes, as well as to determine the effects of the established CDase inhibitors and their activities. In this study, assays for both aCDase and alkCDase activities in fully differentiated human epidermis were optimized using a radiolabeled substrate. These studies revealed that aCDase activity is substantially higher than alkCDase activity, and that both isoenzymes are inhibited by a CDase inhibitor N-oleylethanolamine. These findings were also confirmed using an in situ enzyme assay.


  

Author Contacts

E. Houben
Department of Toxicology, Dermato-Cosmetology and Pharmacognosy
Vrije Universiteit Brussel, Laarbeeklaan 103
BE–1090 Brussels (Belgium)
Tel. +32 2 477 45 96, Fax +32 2 477 45 82, E-Mail evi.houben@vub.ac.be

  

Article Information

Received: October 24, 2006
Accepted: December 22, 2006
Published online: March 29, 2007
Number of Print Pages : 8
Number of Figures : 7, Number of Tables : 0, Number of References : 35

  

Publication Details

Skin Pharmacology and Physiology (Journal of Pharmacological and Biophysical Research)

Vol. 20, No. 4, Year 2007 (Cover Date: June 2007)

Journal Editor: Lademann, J. (Berlin)
ISSN: 1660–5527 (print), 1660–5535 (Online)

For additional information: http://www.karger.com/SPP


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 10/24/2006
Accepted: 12/22/2006
Published online: 3/29/2007

Number of Print Pages: 8
Number of Figures: 7
Number of Tables: 0

ISSN: 1660-5527 (Print)
eISSN: 1660-5535 (Online)

For additional information: http://www.karger.com/SPP


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