Downregulation of Fanconi Anemia Genes in Sporadic Head and Neck Squamous Cell CarcinomaWreesmann V.B. · Estilo C. · Eisele D.W. · Singh B. · Wang S.J.
aDepartment of Otolaryngology – Head and Neck Surgery, University of California, San Francisco, Calif., and bHead and Neck Service, Memorial Sloan-Kettering Cancer Center, New York, N.Y., USA
Background/Aims: Much of our understanding of human cancer has come from studies of the hereditary cancer predisposition syndromes. Fanconi anemia (FA) is an autosomal recessive disorder characterized by cellular hypersensitivity to DNA crosslinking agents, progressive bone marrow failure, and cancer predisposition to solid malignancies, especially head and neck squamous cell carcinoma (HNSCC). Since FA pathway-deficient cells are hypersensitive to DNA crosslinking chemotherapy agents, the presence of somatic FA gene inactivation in sporadic cancers may be of clinical interest. This study sought to determine the frequency of FA gene downregulation in sporadic HNSCC. Methods: The expression of the FA genes FANCA, FANCB, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCJ, FANCL and FANCM in 11 HNSCC cell lines and 49 tongue carcinoma samples was studied with quantitative real-time polymerase chain reaction. Results: Downregulation of at least one FA gene was observed in 3 of 11 HNSCC cell lines and 66% of tongue carcinoma samples. FANCB, FANCF, FANCJ and FANCM were most commonly affected by downregulation, whereas downregulation of FANCA, FANCE and FANCD2 was rare. Conclusion: Our data suggest that downregulation of FA genes is common in sporadic HNSCC. The clinical implications of this finding merit further study.
Steven J. Wang, MD
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Presented at the Annual Meeting of the American Association for Cancer Research, Washington, D.C., April 4, 2006.
Received: August 31, 2006
Accepted after revision: October 14, 2006
Published online: April 4, 2007
Number of Print Pages : 8
Number of Figures : 2, Number of Tables : 3, Number of References : 25
ORL (Journal for Oto-Rhino-Laryngology and Its Related Specialties)
Vol. 69, No. 4, Year 2007 (Cover Date: June 2007)
Journal Editor: O'Malley, B.W., Jr. (Philadelphia, Pa.)
ISSN: 0301–1569 (print), 1423–0275 (Online)
For additional information: http://www.karger.com/ORL