Transport and Metabolism of Serotonin in the Human Placenta from Normal and Severely Pre-Eclamptic PregnanciesCarrasco G. · Cruz M.A. · Gallardo V. · Miguel P. · Dominguez A. · González C.
aDepartment of Physiology, Faculty of Biological Sciences, and bDepartment of Obstetrics, Faculty of Medicine, University of Concepción, Concepción, Chile
We have attempted to elucidate the possible participation of serotonin as an etiological factor in pre-eclampsia. The transport of serotonin into vesicles from the maternal-facing brush border membrane was measured, as well as the metabolism induced by monoamine oxidase (MAO) in placental homogenate obtained from normal-term and severely pre-eclamptic placentas. Kinetic analysis of serotonin uptake by the placental brush border membrane of the syncytiotrophoblast between normally pregnant and severely pre-eclamptic subjects showed no significant difference (similar Vmax and Km values). However, the metabolism of serotonin was significantly higher in placental homogenate from normal pregnancies than in placentas from severely pre-eclamptic pregnancies. These findings suggest that the higher plasma-free serotonin levels observed in severe pre-eclampsia are mainly due to a reduction in MAO-A activity and not limited by the rate of serotonin uptake into the cells.
Department of Physiology, Faculty of Biological Sciences
University of Concepción, PO Box 160-C
Tel. +56 41 29 38 96, Fax +56 41 24 59 75
Received: Received: January 11, 1999
Accepted after revision: October 20, 1999
Number of Print Pages : 6
Number of Figures : 2, Number of Tables : 2, Number of References : 28
Gynecologic and Obstetric Investigation
Founded 1895 as ‘Monatsschrift für Geburtshilfe und Gynäkologie’, continued 1946–1969 as ‘Gynaecologia’ and 1970 –1977 as ‘Gynecologic Investigation’
Vol. 49, No. 3, Year 2000 (Cover Date: Released March 2000)
Journal Editor: G. Zador, Södertälje
ISSN: 0378–7346 (print), 1423–002X (Online)
For additional information: http://www.karger.com/journals/goi