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Table of Contents
Vol. 20, No. 1-4, 2007
Issue release date: January 2006
Section title: Original Paper
Cell Physiol Biochem 2007;20:241–254

Infections with Human Rhinovirus Induce the Formation of Distinct Functional Membrane Domains

Dreschers S. · Franz P.1 · Dumitru C.A.1 · Wilker B.1 · Jahnke K.1 · Gulbins E.1
Department of Molecular Biology and1Department of Otorhinolaryngology, University of Duisburg-Essen
email Corresponding Author

Dr. Stephan Dreschers

Dept. of Molecular Biology, University of Duisburg-Essen

Hufelandstrasse 55, 45122 Essen (Germany)

Tel. +49-201-723-3418, Fax: +49-2001-723-5974

E-Mail stephan.dreschers@uni-essen.de

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The plasma membrane contains distinct domains that are characterized by a high concentration of sphingolipids and cholesterol. These membrane microdomains also referred to as rafts, seem to be intimately involved in transmembranous signaling and often initiate interactions of pathogens and the host cell membranes. Here, we investigated the further reorganization of membrane rafts in cultured epithelial cells and ex vivo isolated nasal cells after infection with rhinoviruses. We demonstrate the formation of ceramide-enriched membrane platforms and large glycosphingolipid-enriched membrane domains and the co-localization of fluorochrome-labeled rhinoviruses with these membrane domains during attachment and uptake of human rhinovirus. Destruction of glycosphingolipid-enriched membrane domains blocked infection of human cells with rhinovirus. Furthermore, our studies indicate that the activation of the acid sphingomyelinase (ASM) is intrigued in the formation of ceramide- or GM1- enriched membrane platforms. Inhibition of the ASM reduces the number of ceramide-enriched platforms and glycosphingolipid-enriched membrane domains. These data reveal a critical role of the ASM for the formation of membrane platforms and infection of human cells with rhinoviruses.

© 2007 S. Karger AG, Basel

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: May 22, 2007
Issue release date: January 2006

Number of Print Pages: 14
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

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