Perinatal Hypoxic/Ischemic Brain Injury Induces Persistent Production of Striatal Neurons from Subventricular Zone ProgenitorsYang Z. · Levison S.W.
a Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, PR China, and b Department of Neurology and Neurosciences, UMDNJ – New Jersey Medical School, Newark, N.J., USA
Ischemia-induced production of new striatal neurons in young and adult rodents has been studied. However, it is unclear whether neonatal hypoxic/ischemic (H/I) brain injury-induced neuronogenesis in the striatum is transient or sustained, nor has it been established whether these new neurons arise from progenitors within the striatum or from precursors residing in the adjacent subventricular zone. Here, we report that from 2 weeks to 5 months after H/I there are more doublecortin-positive (Dcx+) cells and Dcx+/NeuN+ cells in the damaged striatum compared to the contralateral striatum. After the S-phase marker 5-bromo-2′-deoxyuridine (BrdU) was injected at both short and long intervals (2 days and 2 months) after H/I to label newly born cells, more BrdU+/Dcx+ and BrdU+/NeuN+ cells were observed in the ipsilateral striatum compared to the contralateral striatum. Retroviral fate-mapping studies demonstrated that these newly born striatal neurons are generated from precursors within the subventricular zone. Altogether, these observations indicate the neonatal brain initiates a prolonged regenerative response from the precursors of the subventricular zone (SVZ) that results in persistent production of new striatal neurons.
Steven W. Levison, PhD, Laboratory for Regenerative Neurobiology Department of Neurology and Neuroscience and NJMS-UH Cancer Center
UMDNJ – New Jersey Medical School
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Received: December 2, 2006
Accepted after revision: January 31, 2007
Number of Print Pages : 10
Number of Figures : 4, Number of Tables : 0, Number of References : 31
Vol. 29, No. 4-5, Year 2007 (Cover Date: August 2007)
Journal Editor: Campagnoni, A.T. (Los Angeles, Calif.)
ISSN: 0378–5866 (print), 1421–9859 (Online)
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