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Table of Contents
Vol. 14, No. 1-3, 2008
Issue release date: October 2007
Section title: Paper
Free Access
J Mol Microbiol Biotechnol 2008;14:6–15

Molecular Characterization of Intrinsic and Acquired Antibiotic Resistance in Lactic Acid Bacteria and Bifidobacteria

Ammor M.S.a · Flórez A.B.a · van Hoek A.H.A.M.b · de los Reyes-Gavilán C.G.a · Aarts H.J.M.b · Margolles A.a · Mayo B.a
aInstituto de Productos Lácteos de Asturias (IPLA), Consejo Superior de Investigaciones Científicas (CSIC), Carretera de Infiesto s/n, Villaviciosa, Spain; bRIKILT – Institute of Food Safety, Wageningen UR, Wageningen, The Netherlands
email Corresponding Author

Mohammed Salim Ammor

Instituto de Productos Lácteos de Asturias (CSIC)

Carretera de Infiesto s/n

ES–33300 Villaviciosa (Spain)

Tel. +34 985 89 12 31, Fax +34 985 89 22 33, E-Mail ammor@ipla.csic.es

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The minimum inhibitory concentrations (MICs) of 6 different antibiotics (chloramphenicol, clindamycin, erythromycin, streptomycin, tetracycline and vancomycin) were determined for 143 strains of lactic acid bacteria and bifidobacteria using the Etest. Different MICs were found for different species and strains. Based on the distribution of these MIC values, most of the strains were either susceptible or intrinsically resistant to these antibiotics. However, the MIC range of some of these antibiotics showed a bimodal distribution, which suggested that some of the tested strains possess acquired antibiotic resistance. Screening for resistance genes was performed by PCR using specific primers, or using a DNA microarray with around 300 nucleotide probes representing 7 classes of antibiotic resistance genes. The genes identified encoded resistance to tetracycline [tet(M), tet(W), tet(O) and tet(O/W)], erythromycin and clindamycin [erm(B)] and streptomycin [aph(E) and sat(3)]. Internal portions of some of these determinants were sequenced and found to be identical to genes described in other bacteria. All resistance determinants were located on the bacterial chromosome, except for tet(M), which was identified on plasmids in Lactococcus lactis. The contribution of intrinsic multidrug transporters to the antibiotic resistance was investigated by cloning and measuring the expression of Bifidobacterium breve genes in L. lactis.

© 2008 S. Karger AG, Basel

Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: October 24, 2007
Issue release date: October 2007

Number of Print Pages: 10
Number of Figures: 1
Number of Tables: 5

ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

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