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Table of Contents
Vol. 75, No. 4, 2008
Issue release date: May 2008
Section title: Clinical Investigations
Respiration 2008;75:437–442
(DOI:10.1159/000107741)

Pleural Fluid PCR Method for Detection of Staphylococcus aureus, Streptococcus pneumoniae and Haemophilus influenzae in Pediatric Parapneumonic Effusions

Utine G.E.a · Pinar A.b · Özçelik U.a · Şener B.b · Yalçin E.a · Doğru D.a · Menemenlioğlu D.b · Gür D.c · Kiper N.a · Göçmen A.a
aDepartment of Pediatrics, Division of Chest Diseases, bDepartment of Microbiology and Clinical Microbiology, and cMicrobiology Laboratory, Ihsan Dogramaci Children’s Hospital, Hacettepe University, Faculty of Medicine, Sıhhiye/Ankara, Turkey

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Article / Publication Details

First-Page Preview
Abstract of Clinical Investigations

Received: January 09, 2007
Accepted: June 07, 2007
Published online: August 29, 2007
Issue release date: May 2008

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 2

ISSN: 0025-7931 (Print)
eISSN: 1423-0356 (Online)

For additional information: http://www.karger.com/RES

Abstract

Background: Parapneumonic effusions cause significant morbidity and mortality despite current developments in diagnostic and therapeutic approaches. Causative microorganisms may remain unidentified in a significant number of patients by cultures and Gram smears. Polymerase chain reaction (PCR) is a molecular technique for the detection of causative bacteria; however, its efficiency in pleural fluids is less known. Objectives: The present study was performed to compare the efficiency of PCR in the detection of the three most common organisms (Staphylococcus aureus, Streptococcus pneumoniae and Haemophylus influenzae) with conventional methods. Methods: Twenty-eight consecutive patients with parapneumonic pleural effusions were studied. On admission, pleural fluid samples were obtained for Gram staining, routine culture and PCR analysis for S. aureus, S. pneumoniae and H. influenzae. Results: PCR analysis allowed detection of 11 microorganisms in 10 patients (35.7%), whereas pleural fluid cultures detected the etiological agent in only 2 (7.1%). S. pneumoniae was the most frequent agent. Conclusions: Pleural fluid cultures may have low diagnostic yields, partly due to prior antibiotic use. Pleural fluid PCR analysis may improve the etiologic diagnosis in parapneumonic pleural effusions, with technical advances leading to higher yields than obtained in this study.

© 2007 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Clinical Investigations

Received: January 09, 2007
Accepted: June 07, 2007
Published online: August 29, 2007
Issue release date: May 2008

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 2

ISSN: 0025-7931 (Print)
eISSN: 1423-0356 (Online)

For additional information: http://www.karger.com/RES


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