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Vol. 41, No. 6, 2007
Issue release date: November 2007
Section title: Original Paper
Caries Res 2007;41:467–473
(DOI:10.1159/000107934)

A New in vitro Model to Study the Relationship of Gap Size and Secondary Caries

Totiam P.a · González-Cabezas C.b · Fontana M.R.b · Zero D.T.b
aFaculty of Dentistry, Naresuan University, Phitsanulok, Thailand; bIndiana University School of Dentistry, Indianapolis, Ind., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 4/3/2007
Accepted: 5/31/2007
Published online: 9/7/2007
Issue release date: November 2007

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 2

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE

Abstract

Objectives: To investigate the relationship of gap size and secondary caries in a newly developed secondary caries microbial model that permits adjustment of the gap between the tooth and a restoration. Methods: Tooth-resin-matrix composite specimens were mounted on gap-model stages with a gap size of 50 or 500 µm in experiment 1, and 0, 25, 250, or 1,000 µm in experiment 2. They were attached to plastic Petri plates, gas-sterilized and then incubated in a microbial caries model (with Streptococcus mutans TH16 in 1% sucrose tryptic soy broth for 1 h, 4 times/day, and with a buffer solution for the rest of the day). After 8 days of incubation, tooth specimens were sectioned and stained overnight with a rhodamine B solution. Digital images taken under a confocal microscope were analyzed for lesion size at the outer surface lesion and wall lesion (WL). Results: Gap size was found to affect the development of dentin WL area in experiment 1 and enamel and dentin WL areas in experiment 2, with bigger lesions being observed in the wider gap group (p < 0.05). Conclusion: The findings of this study suggest that the size of the gap between tooth and restoration affects the development of secondary caries along the cavity wall.

© 2007 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 4/3/2007
Accepted: 5/31/2007
Published online: 9/7/2007
Issue release date: November 2007

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 2

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE


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