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Vol. 145, No. 3, 2008
Issue release date: February 2008
Section title: Original Paper
Int Arch Allergy Immunol 2008;145:182–192
(DOI:10.1159/000109287)

A Comparison of Inflammatory Mediators Released by Basophils of Asthmatic and Control Subjects in Response to High-Affinity IgE Receptor Aggregation

Gilmartin L.a · Tarleton C.A.a · Schuyler M.b · Wilson B.S.a · Oliver J.M.a
Departments of aPathology and bMedicine, University of New Mexico, Albuquerque, N.Mex., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 10/24/2006
Accepted: 5/30/2007
Published online: 10/2/2007
Issue release date: February 2008

Number of Print Pages: 11
Number of Figures: 7
Number of Tables: 3

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Background: In human blood basophils, cross-linking the high-affinity IgE receptor FcεRI with multivalent antigen activates a signaling pathway leading to secretion of inflammatory mediators and cytokine production. Basophils are known to play an important role in the pathogenesis of asthma but there has been no comprehensive examination of the effectors these cells produce. Here a study of the transcription and release of a selection of chemokines and cytokines from basophils was undertaken. Methods: A Cartesian antibody array provided an effective method of assaying for multiple cytokines and chemokines simultaneously. Results were verified by RT-PCR and ELISA assays. This allowed the comparison of freshly prepared peripheral blood basophil responses to cross-linking of the high-affinity IgE receptor, with and without preincubation with IL-3. Results: Evidence that human blood basophils produce the chemokines MIP-5, eotaxin and GM-CSF was provided by antibody array and RT-PCR analyses. Preincubation with IL-3 enhanced the expression and release of IL-13, IL-8 and mRNA transcripts encoding MIP-5 and GATA2 in basophils from both asthmatic and control subjects. Leptin mRNA transcription, storage and release in basophils are described for the first time. Conclusions: Surveying cytokine and chemokines stored and released by peripheral blood basophils shows that asthmatic and control subjects share similar profiles even when their degranulation responses are distinct. Evidence is provided for the production of leptin, GM-CSF, eotaxin and MIP-5 by peripheral blood basophils. IL-3 preincubation enhances the production and release of IL-8 upon IgE receptor cross-linking.

© 2007 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 10/24/2006
Accepted: 5/30/2007
Published online: 10/2/2007
Issue release date: February 2008

Number of Print Pages: 11
Number of Figures: 7
Number of Tables: 3

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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