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Vol. 187, No. 2, 2008
Issue release date: February 2008
Section title: Original Paper
Cells Tissues Organs 2008;187:89–102
(DOI:10.1159/000109946)

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

Hao W. · Hu Y.-Y. · Wei Y.-Y. · Pang L. · Lv R. · Bai J.-P. · Xiong Z. · Jiang M.
aInstitute of Orthopaedics, Xijing Hospital, and bDepartment of Oral Histology and Pathology, College of Stomatology, Fourth Military Medical University, Xi’an; cDepartment of Mechanical Engineering, Tsinghua University, Beijing, PR China

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: 5/25/2007
Published online: 10/15/2007

Number of Print Pages: 14
Number of Figures: 9
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

Cell-based tissue engineering is thought to be a new therapy for treatment of bone defects and nonunions after trauma and tumor resection. In this study, we explore the in vitro and in vivo osteogenesis of a novel biomimetic construct fabricated by using collagen I gel to suspend rabbit adipose-derived stem cells (rASCs) into a porous poly(lactic-co-glycolic)acid-β-tricalcium phosphate (PLGA-β-TCP) scaffold (rASCs-COL/PLGA-β-TCP). In vitro and in vivo studies of the rASCs-COL/PLGA-β-TCP composite (group A) were carried out compared with the single combination of rASCs and PLGA-β-TCP (rASCs/PLGA-β-TCP; group B), the combination of acellular collagen I gel and PLGA-β-TCP (COL/PLGA-β-TCP; group C), and the PLGA-β-TCP scaffold (group D). Composites of different groups were cultured in vitro for 2 weeks in osteogenic medium and then implanted into the autologous muscular intervals for 8 weeks. After 2 weeks of in vitro culture, alkaline phosphatase activity and extracellular matrix mineralization in group A were significantly higher than in group B (p < 0.01, n = 4). In vivo osteogenesis was evaluated by radiographic and histological analyses. The calcification level was radiographically evident in group A, whereas no apparent calcification was observed in groups B, C and D (n = 4). In group A, woven bone with a trabecular structure was formed, while in group B, only osteoid tissue was observed. Meanwhile, the bone-forming area in group A was significantly higher than in group B (p < 0.01, n = 4). No bone formation was observed in groups C or D (n = 4). In conclusion, by using collagen I gel to suspend rASCs into porous PLGA-β-TCP scaffold, osteogenic differentiation of rASCs can be improved and homogeneous bone tissue can be successfully formed in vivo.


  

Author Contacts

Dr. Yun-Yu Hu
Institute of Orthopaedics, Xijing Hospital
Fourth Military Medical University, 15 Changlexi Street
Xi’an 710032, Shannxi (PR China)
Tel. +86 298 477 5291, Fax +86 298 477 5573, E-Mail ehaw@163.com

  

Article Information

Accepted after revision: May 25, 2007
Published online: October 15, 2007
Number of Print Pages : 14
Number of Figures : 9, Number of Tables : 1, Number of References : 61

  

Publication Details

Cells Tissues Organs (in vivo, in vitro)

Vol. 187, No. 2, Year 2008 (Cover Date: February 2008)

Journal Editor: Denker, H.-W. (Essen), English, A.W. (Atlanta, Ga.)
ISSN: 1422–6405 (Print), eISSN: 1422–6421 (Online)

For additional information: http://www.karger.com/CTO


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: 5/25/2007
Published online: 10/15/2007

Number of Print Pages: 14
Number of Figures: 9
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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