In vivo Adipose Tissue Regeneration by Adipose-Derived Stromal Cells Isolated from GFP Transgenic MiceMizuno H. · Itoi Y. · Kawahara S. · Ogawa R. · Akaishi S. · Hyakusoku H.
Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan
We have previously demonstrated that pluripotent stem cells can be obtained from green fluorescence protein (GFP) transgenic mouse adipose tissue. In this study, we sought to determine whether adipose tissue regeneration can be induced in vivo using adipose-derived stromal cells (ASCs) from GFP mice. ASCs were isolated from inguinal fat pads of GFP mice, as described in our previous publication. After incubation in two passages in the control medium, the cells were incubated in the induction medium to induce adipogenesis. Induced ASCs were merged with fibrin glue, and the mixture was injected subcutaneously into the dorsum of athymic mice. Finally, specimens were harvested and analyzed morphologically and histologically. The regenerated tissue was macroscopically semitransparent and soft with slight angiogenesis. Fluorescence microscopy revealed that the specimens strongly emitted green fluorescence, suggesting that the transplanted ASCs had contributed to adipogenesis. Both hematoxylin and eosin and oil red O staining revealed that cells containing small lipid droplets had been regenerated histologically. These findings suggest that ASCs could contribute to adipose tissue regeneration in vivo. ASCs may be an ideal source for adipose tissue regeneration, which may in turn play an important role in augmentation surgery in surgically treated cancer or trauma patients.
© 2007 S. Karger AG, Basel
Dr. Hiroshi Mizuno
Department of Plastic and Reconstructive Surgery, Nippon Medical School
1-1-5 Sendagi, Bunkyo-ku
Tokyo 1138603 (Japan)
Tel. +81 3 5814 6208, Fax +81 3 5685 3076, E-Mail email@example.com
Presented at the 2nd International Fat Applied Technology Society Meeting, Pittsburgh, Pa., USA, 2004, and the Joint Meeting of the Tissue Engineering Society International and the European Tissue Engineering Society, Lausanne, 2004.
Accepted after revision: August 10, 2007
Published online: November 2, 2007
Number of Print Pages : 9
Number of Figures : 6, Number of Tables : 1, Number of References : 39
Cells Tissues Organs (in vivo, in vitro)
Vol. 187, No. 3, Year 2008 (Cover Date: March 2008)
Journal Editor: Denker H.-W. (Essen), English A.W. (Atlanta, Ga.)
ISSN: 1422–6405 (Print), eISSN: 1422–6421 (Online)
For additional information: http://www.karger.com/CTO