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Table of Contents
Vol. 187, No. 3, 2008
Issue release date: March 2008
Section title: Original Paper
Cells Tissues Organs 2008;187:177–185
(DOI:10.1159/000110805)

In vivo Adipose Tissue Regeneration by Adipose-Derived Stromal Cells Isolated from GFP Transgenic Mice

Mizuno H. · Itoi Y. · Kawahara S. · Ogawa R. · Akaishi S. · Hyakusoku H.
Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: August 10, 2007
Published online: November 02, 2007
Issue release date: March 2008

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

We have previously demonstrated that pluripotent stem cells can be obtained from green fluorescence protein (GFP) transgenic mouse adipose tissue. In this study, we sought to determine whether adipose tissue regeneration can be induced in vivo using adipose-derived stromal cells (ASCs) from GFP mice. ASCs were isolated from inguinal fat pads of GFP mice, as described in our previous publication. After incubation in two passages in the control medium, the cells were incubated in the induction medium to induce adipogenesis. Induced ASCs were merged with fibrin glue, and the mixture was injected subcutaneously into the dorsum of athymic mice. Finally, specimens were harvested and analyzed morphologically and histologically. The regenerated tissue was macroscopically semitransparent and soft with slight angiogenesis. Fluorescence microscopy revealed that the specimens strongly emitted green fluorescence, suggesting that the transplanted ASCs had contributed to adipogenesis. Both hematoxylin and eosin and oil red O staining revealed that cells containing small lipid droplets had been regenerated histologically. These findings suggest that ASCs could contribute to adipose tissue regeneration in vivo. ASCs may be an ideal source for adipose tissue regeneration, which may in turn play an important role in augmentation surgery in surgically treated cancer or trauma patients.

© 2007 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Accepted: August 10, 2007
Published online: November 02, 2007
Issue release date: March 2008

Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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