Background: Studies performed in 5 laboratories have
shown that homeopathically prepared highly diluted thyroxin
(10-30 = 30x) slowed down metamorphosis of highland
amphibians. Metamorphosis of lowland amphibians, however,
could be slowed down by a low dilution of thyroxin
(10-8 = 8x) if animals had been artificially pretreated with
thyroxin. Objective: To combine the advantages of using animals
from highland biotopes and hyperstimulation prior to
treatment. Animals and Methods: Rana temporaria from an
alpine biotope were pretreated in an aqueous molecular
thyroxin dilution (10-8 parts by weight, hyperstimulation).
This is supposed to accelerate metamorphosis. In accordance
with the homeopathic idea of detoxification or cure,
one group of these hyperstimulated animals was then treated
with thyroxin 30x, and another group with water 30x. Experiments
were performed by 4 independent researchers.
Results: As a trend, the thyroxin-30x animals metamorphosed
more slowly than the water-30x animals. The number
of thyroxin-30x animals that reached the 4-legged stage
at defined points in time was slightly smaller at some but
not all points in time, compared to control. This is in line
with previous findings and can be discussed as an interesting
result. Contrary to our working hypothesis, however, differences
were not bigger than in the previous experiments
in which animals had not been pretreated with thyroxin
10-8. Conclusion: This study supports previous findings but
does not prove the assumption that pretreatment of highland
animals with molecular thyroxine improves the original
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