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Table of Contents
Vol. 119, No. 3-4, 2007
Issue release date: February 2008
Section title: Original Article
Cytogenet Genome Res 119:204–210 (2007)
(DOI:10.1159/000112062)

U-HO1, a new cell line derived from a primary refractory classical Hodgkin lymphoma

Mader A.a · Brüderlein S.b · Wegener S.b · Melzner I.b · Popov S.b · Müller-Hermelink H.K.d · Barth T.F.b · Viardot A.c · Möller P.b
aHappareute, Röthenbach; bInstitute of Pathology and cDepartment of Hematology and Oncology, University of Ulm, Ulm; dInstitute of Pathology, University of Würzburg, Würzburg (Germany)

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: September 25, 2007
Published online: February 01, 2008
Issue release date: February 2008

Number of Print Pages: 7
Number of Figures: 5
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

The Hodgkin cell line U-HO1 was established from a malignant pleural effusion of a 23-year-old male patient during the end stage of refractory nodular sclerosing classical Hodgkin lymphoma (cHL). Since its establishment in 2005, U-HO1 has maintained stable characteristics in vitro and has a doubling time of about 4 days under standard culture conditions. U-HO1 forms typical Reed-Sternberg cells in suspension, is EBV negative, lacks HLA-A, -B, -C but expresses HLA-D proteins/CD74 and exposes CD15 together with CD30 in the absence of CD19 and CD20 on the cell surface. Karyotype analysis of U-HO1 revealed a hyperdiploid karyotype with multiple clonal aberrations. Most significant is an elongated chromosome 2, der(2)t(2;10)(q35; q16.1)add(2)(p13). CGH analysis revealed the following imbalances: ish cgh dim(1)(p13p31)(p12q21), enh(2)(p13p23), dim(4)(q31.3qter), enh(6)(q22q27), enh(12), enh(18), enh(20) (q13.1pter). FISH analysis showed about six-fold amplification of REL and BCL11A, thus, U-HO1 is prototypical for cHL in every aspect tested so far. As an outstanding feature compared to the existing HL cell lines, U-HO1 has high levels of microRNA transcripts of MIRN216 and MIRN217 located in the amplicon 2p16. Compared to other HL cell lines, U-HO1 proved far less genetically aberrant suggesting that U-HO1’s imbalances suffice to cause the full-blown phenotype of primary refractory cHL.

© 2008 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: September 25, 2007
Published online: February 01, 2008
Issue release date: February 2008

Number of Print Pages: 7
Number of Figures: 5
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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