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Vol. 16, No. 3-4, 2009
Issue release date: February 2009
Section title: Research Article
J Mol Microbiol Biotechnol 2009;16:146–158
(DOI:10.1159/000115849)

PilR, a Transcriptional Regulator for Pilin and Other Genes Required for Fe(III) Reduction in Geobacter sulfurreducens

Juárez K. · Kim B.-C. · Nevin K. · Olvera L. · Reguera G. · Lovley D.R. · Methé B.A.
aDepartment of Microbiology, University of Massachusetts Amherst, Amherst, Mass., bDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Mich., cThe Institute for Genomic Research, Rockville, Md., USA; dDepartamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, México

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Article / Publication Details

First-Page Preview
Abstract of Research Article

Published online: 2/4/2008

Number of Print Pages: 13
Number of Figures: 6
Number of Tables: 4

ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

For additional information: http://www.karger.com/MMB

Abstract

Growth using Fe(III) as a terminal electron acceptor is a critical physiological process in Geobacter sulfurreducens. However, the mechanisms of electron transfer during Fe(III) reduction are only now being understood. It has been demonstrated that the pili in G. sulfurreducens function as microbial nanowires conducting electrons onto Fe(III) oxides. A number of c-type cytochromes have also been shown to play important roles in Fe(III) reduction. However, the regulatory networks controlling the expression of the genes involved in such processes are not well known. Here we report that the expression of pilA, which encodes the pilistructural protein, is directly regulated by a two-component regulatory system in which PilR functions as an RpoN-dependent enhancer binding protein. Surprisingly, a deletion of the pilR gene affected not only insoluble Fe(III) reduction, which requires pili, but also soluble Fe(III) reduction, which, in contrast, does not require pili. Gene expression profiling using whole-genome DNA microarray and quantitative RT-PCR analyses obtained with a PilR-deficient mutant revealed that the expression of pilA and other pilin-related genes are downregulated, while many c-type cytochromes involved in Fe(III) reduction were differentially regulated. This is the first instance of an enhancer binding protein implicated in regulating genes involved in Fe(III) respiratory functions.


  

Author Contacts

Katy Juárez
Departamento de Ingeniería Celular y Biocatálisis
Instituto de Biotecnología, Universidad Nacional Autónoma de México
Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Mor. CP 62210 (México)
Tel./Fax +52 777 3291 601, E-Mail katy@ibt.unam.mx

  

Article Information

Published online: February 4, 2008
Number of Print Pages : 13
Number of Figures : 6, Number of Tables : 4, Number of References : 44

  

Publication Details

Journal of Molecular Microbiology and Biotechnology

Vol. 16, No. 3-4, Year 2009 (Cover Date: February 2009)

Journal Editor: Saier Jr. M.H. (La Jolla, Calif.)
ISSN: 1464-1801 (Print), eISSN: 1660-2412 (Online)

For additional information: http://www.karger.com/MMB


Article / Publication Details

First-Page Preview
Abstract of Research Article

Published online: 2/4/2008

Number of Print Pages: 13
Number of Figures: 6
Number of Tables: 4

ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

For additional information: http://www.karger.com/MMB


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