Basophil Sensitivity in Patients Not Responding to Venom ImmunotherapyPeternelj A. · Silar M. · Erzen R. · Kosnik M. · Korosec P.
University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia
Background: Treatment failure of venom immunotherapy (VIT) is not rare and the risk and pathogenic factors for those failures are so far poorly understood. For that reason we evaluated allergen-specific basophil sensitivity in patients who did not tolerate field re-stings after completed VIT treatment. Methods: Basophil responsiveness was evaluated by flow cytometry analyses of basophil CD63 surface expression induced by different concentrations of bee or wasp venom (1, 0.1 and 0.01 µg/ml) in 14 treated patients who had experienced systemic allergic reactions (Muller grades II–III) and 17 treated patients who had no reactions after the field re-stings. We also included a group of 28 Hymenoptera venom-allergic patients who had not received VIT. Results: In 14 patients who still reacted to bee or wasp sting, basophil response at a venom concentration of 0.1 µg/ml was significantly higher than in patients who tolerated field re-stings (p = 0.03; t test). Basophil response was also slightly higher at a concentration of 1 µg/ml, but not to statistical significance (p = 0.12; t test). There was no difference in the response to direct cross-linking of the IgE and in venom-specific IgE and IgG4 serum concentrations between those 2 groups (p > 0.8; Fisher’s exact test, t test). Patients who tolerated field re-stings have also significantly lower basophil response in comparison to patients who had not received VIT, both at 0.1 and 1 µg/ml of venom concentrations (p < 0.001; t test). Conclusions: The results suggest that basophil venom-specific sensitivity is associated with the efficiency of VIT.
© 2008 S. Karger AG, Basel
Received: April 12, 2007
Accepted after revision: October 19, 2007
Published online: February 13, 2008
Number of Print Pages : 7
Number of Figures : 3, Number of Tables : 2, Number of References : 24
International Archives of Allergy and Immunology
Vol. 146, No. 3, Year 2008 (Cover Date: June 2008)
Journal Editor: Valenta, R. (Vienna)
ISSN: 1018–2438 (Print), eISSN: 1423–0097 (Online)
For additional information: http://www.karger.com/IAA