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Vol. 45, No. 5, 2008
Issue release date: August 2008
Section title: Research Paper
J Vasc Res 2008;45:365–374
(DOI:10.1159/000121406)

Antisense to Transforming Growth Factor-β1 Facilitates the Apoptosis of Macrophages in Rat Vein Grafts

Heaton N.S. · Wolff R.A. · Malinowski R.L. · Hullett D.A. · Hoch J.R.
W.S. Middleton VA Hospital and the University of Wisconsin School of Medicine and Public Health, Madison, Wisc., USA

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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 8/10/2007
Accepted: 12/4/2007
Published online: 3/20/2008

Number of Print Pages: 10
Number of Figures: 3
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR

Abstract

Background: The success of peripheral vein grafts is limited by intimal hyperplasia. Transforming growth factor (TGF)-β1 has effects on cell proliferation, apoptosis and extracellular matrix synthesis. We have previously observed positive changes in vessel healing with antisense to TGF-β1. Methods: Adenovirus was used to transduce rat femoral artery vein grafts with antisense to TGF-β1 (Ad-AST) or the sequence encoding the bioactive form of TGF-β1 (Ad-BAT). Grafts were harvested at 1, 2, 4 and 12 weeks and formalin fixed for immunohistochemical studies of the cell markers proliferating cellular nuclear antigen (proliferation) and active caspase 3 (apoptosis). In situ DNA fragmentation assays were also performed to confirm active caspase 3 results. Results: Ad-AST treatment significantly (p = 0.05) increased apoptosis of macrophages inside the internal elastic lamina. In addition, Ad-AST treatment significantly increased the cellularity of the graft at early time points and reduced it at later time points (p = 0.01). Conclusion: The low levels of TGF-β1 in Ad-AST treatment promote apoptosis of macrophages and provide an environment that is more conducive to the proliferation or infiltration of cells that contribute to healthy vessels.


  

Author Contacts

Dr. Randal Wolff
W.S. Middleton VA Hospital
2500 Overlook Terrace, G-wing Rm G9
Madison, WI 53705 (USA)
Tel. +1 608 265 9143, Fax +1 608 265 9144, E-Mail wolff@surgery.wisc.edu

  

Article Information

Received: August 10, 2007
Accepted after revision: December 4, 2007
Published online: March 20, 2008
Number of Print Pages : 10
Number of Figures : 3, Number of Tables : 1, Number of References : 31

  

Publication Details

Journal of Vascular Research (Incorporating 'International Journal of Microcirculation')

Vol. 45, No. 5, Year 2008 (Cover Date: August 2008)

Journal Editor: Pohl U. (Munich), Meininger G.A. (Columbia, Mo.)
ISSN: 1018–1172 (Print), eISSN: 1423–0135 (Online)

For additional information: http://www.karger.com/JVR


Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 8/10/2007
Accepted: 12/4/2007
Published online: 3/20/2008

Number of Print Pages: 10
Number of Figures: 3
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR


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