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Table of Contents
Vol. 29, No. 2, 1979
Issue release date: 1979
Section title: Original Paper
Neuroendocrinology 1979;29:84–89
(DOI:10.1159/000122909)

Characterization of a Cytosol Progesterone Receptor in Bovine Pineal Gland

Vacas M.I. · Lowenstein P.R. · Cardinali D.P.
Centro de Estudios Farmacológicos y de Principios Naturales (CEFAPRIN), Buenos Aires

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 11, 1978
Accepted: January 08, 1979
Published online: March 26, 2008
Issue release date: 1979

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)

For additional information: http://www.karger.com/NEN

Abstract

The possible existence of pineal progesterone (P) receptors was assessed in 105,000 g supernatants of bovine pineal glands incubated with 3H-P in the presence of 10 μM cortisol. By charcoal adsorption analysis specific binding (defined as the difference between 3H-P binding in the absence and presence of 1,000-fold excess of unlabeled P) attained its maximum after 60 min at 37 °C and after 5 h at 0 °C. Binding was abolished by heating the cytosol or by pre-incubating it with trypsin. A single population of binding sites with a Kd = 1.64 nM and binding site concentration = 34 fmol/mg of cytosol protein was detectable by Scatchard analysis. In competition experiments the B50 progesterone values (nM) were: P (2), 20α-dihydroprogesterone (12), 20β-dihydroprogesterone (25), estradiol (158), testosterone (675) and corticosterone (> 1,000). After sucrose gradient centrifugation of pineal cytosol incubated with 3H-P a discrete peak of radioactivity in the 7-8 S region of the gradient was observed, and disappeared following incubation with excess unlabeled P. These results indicate that P receptors are present in the bovine pineal gland.

© 1979 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 11, 1978
Accepted: January 08, 1979
Published online: March 26, 2008
Issue release date: 1979

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)

For additional information: http://www.karger.com/NEN


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