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Vol. 121, No. 1, 2008
Issue release date: June 2008
Section title: Original Article
Cytogenet Genome Res 121:18–24 (2008)
(DOI:10.1159/000124377)

Karyotype analysis of the euploid cell population of a mouse embryonic stem cell line revealed a high incidence of chromosome abnormalities that varied during culture

Rebuzzini P. · Neri T. · Mazzini G. · Zuccotti M. · Redi C.A. · Garagna S.
aDipartimento di Biologia Animale, Laboratorio di Biologia dello Sviluppo, Università degli Studi di Pavia, bIstituto di Genetica Molecolare del CNR, Sezione di Istochimica e Citometria, Dipartimento di Biologia Animale, Pavia, cDipartimento di Medicina Sperimentale, Sezione di Istologia ed Embriologia, Università degli Studi di Parma, Parma, dFondazione IRCCS Policlinico San Matteo, Pavia (Italy)

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Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: 12/14/2007
Published online: 5/7/2008

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

It is common knowledge that mouse embryonic stem cell (mESC) lines accumulate chromosomal changes during culture. Despite the wide use of mESCs as a model of early mammalian development and cell differentiation, there is a lack of systematic studies aimed at characterizing their karyological changes during culture. We cultured an mESC line, derived in our laboratory, for a period of 3 months investigating its chromosome complement at different times. About 60% of the metaphases analysed were euploid throughout the culture period but, from passage 13, only 50% of the euploid metaphases had a proper chromosome complement. The remaining 50% showed chromosome abnormalities, mainly gain or loss of entire chromosomes, both within the same passage and among different passages analysed. The very heterogeneous spectrum of abnormalities indicates a high frequency of chromosome mutations that arise continuously during culture. The heterogeneity of the aberrant chromosome constitution of 2n = 40 metaphases, observed at different passages of culture, might be due either to their elimination or to a shift towards the hypoeu- or hypereuploid population of those metaphases that accumulate further chromosome abnormalities. The stability of the frequency of eu-, hypoeu- and hypereuploid populations during culture might, however, be due to the elimination of those cells that carry a high mutational burden. Based on our results, we suggest that karyotype analysis of the euploid cell population of mESC lines is necessary when such lines are used in the production of chimeric mice, for their contribution to the germ line, or when they are differentiated into specific cell types.


  

Author Contacts

Request reprints from Silvia Garagna
Dipartimento di Biologia Animale, Laboratorio di Biologia dello Sviluppo
Piazza Botta 9, Universita’ degli Studi di Pavia, IT–27100 Pavia (Italy)
telephone: +39 038 2986 323; fax: +39 038 2986 270
e-mail: silvia.garagna@unipv.it

  

Article Information

This work was supported by grants from PRIN-COFIN 2005, FIRB 2005 (Project N. RBIP06FH7J), Istituto Superiore di Sanita’ (Programma Nazionale Cellule Staminali 2003–2004), Millipore, and Olympus Foundation Science for Life.

Accepted in revised form for publication by M. Schmid,: 14 December 2007.
Published online: May 07, 2008
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 2, Number of References : 40

  

Publication Details

Cytogenetic and Genome Research

Vol. 121, No. 1, Year 2008 (Cover Date: June 2008)

Journal Editor: Schmid M. (Würzburg)
ISSN: 1424–8581 (Print), eISSN: 1424–859X (Online)

For additional information: http://www.karger.com/CGR


Article / Publication Details

First-Page Preview
Abstract of Original Article

Accepted: 12/14/2007
Published online: 5/7/2008

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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