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Mesenchymal Progenitor Cells: Tissue Origin, Isolation And CultureBourin P.a · Gadelorge M.a · Peyrafitte J.b · Fleury-Cappellesso S.a · Gomez M.a · Rage C.a · Sensebe L.c
a Laboratoire d’Ingénierie cellulaire, GECSoM, Etablissement Français du Sang Pyrénées-Méditerranée, bUMR CNRS UPS 5241, Toulouse, c Laboratoire de recherche, GECSoM, Etablissement Français du Sang Centre-Atlantique, Tours, France Corresponding Author
Dr. Philippe Bourin, Laboratoire d’Ingénierie cellulaire, Etablissement Français du Sang Pyrénées-Méditerranée, 75 rue de Lisieux, 31300 Toulouse, France, Tel. +33 534 5024-78, Fax -70, E-mail firstname.lastname@example.org
Since the pioneering work of Alexander Friedenstein on multipotent mesenchymal stromal cells (MSCs), a tremendous amount of work has been done to isolate, characterize and culture such cells. Assay of colony forming unit-fibroblasts (CFU-Fs), the hallmark of MSCs, is used to estimate their frequency in tissue. MSCs are adherent cells, so they are easy to isolate, and they show contact inhibition. Thus, several parameters must be taken into account for culture: cell density, number of passages, culture medium, and growth factors used. The purity of the initial material is not a limiting parameter. Similar but not identical cell populations are found in almost all mammal or human tissues. MSCs seem to be very abundant in adipose tissue but at low frequency in blood from umbilical cord or in adult tissue. The culture conditions are very similar, whatever the source of cells. Because of their favorable properties, MSCs are very promising tools for regenerative medicine.
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