Cover

Proteomics in Nephrology - Towards Clinical Applications

Editor(s): Thongboonkerd V. (Bangkok) 
Table of Contents
Vol. 160, No. , 2008
Section title: Paper
Thongboonkerd V (ed): Proteomics in Nephrology - Towards Clinical Applications. Contrib Nephrol. Basel, Karger, 2008, vol 160, pp 172-185
(DOI:10.1159/000125981)

Proteomic Approaches for the Study of Cell Signaling in the Renal Collecting Duct

Hoorn E. · Pisitkun T. · Yu M. · Knepper M.
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md., USA

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 4/4/2008
Cover Date: 2008

Number of Print Pages: 14
Number of Figures: 0
Number of Tables: 0

ISBN: 978-3-8055-8544-6 (Print)
eISBN: 978-3-8055-8545-3 (Online)

Abstract

In the current era of large-scale biology, proteomics has evolved as a powerful, new technique that aims to identify, quantify, and analyze a large number of proteins in a functional context. Therefore, proteomics can be used to study cellular pathways and identify disease biomarkers. In this review, we first outline the principles of two important proteomics techniques that either use difference gel electrophoresis (DIGE) or liquid chromatography (LC) for protein separation, followed by tandem mass spectrometry (MS/MS). The advantages and limitations of each technique are discussed, emphasizing the ability of DIGE to perform quantitative proteomics and the high-throughput and high-sensitivity characteristics of LC-MS/MS. We have employed both techniques to unravel the molecular machinery of vasopressin signaling, which governs water homeostasis by recruiting aquaporin-2 (AQP2) water channels after activation of the vasopressin-2 receptor by vasopressin. Several aspects of vasopressin signaling in the inner medullary collecting duct (IMCD) were investigated, including the short- and long-term regulation of AQP2, phosphoproteomics, signaling during vasopressin escape, and the proteomes of AQP2-bearing vesicles and the IMCD plasma membranes. We also emphasize that proteomics of body fluids will be the strategy to identify disease biomarkers, and therefore conclude the review by highlighting the perspectives of biomarker discovery in urinary exosomes.


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 4/4/2008
Cover Date: 2008

Number of Print Pages: 14
Number of Figures: 0
Number of Tables: 0

ISBN: 978-3-8055-8544-6 (Print)
eISBN: 978-3-8055-8545-3 (Online)


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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