A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatinWreggett K.A. · Hill F. · James P.S. · Hutchings A. · Butcher G.W. · Singh P.B.
aCell Determination Group, Department of Development and Signalling, bMonoclonal Antibody Unit, Department of Immunology, and cConfocal Microscope Facility, Department of Cellular Physiology, AFRC Babraham Institute, Babraham, Cambridge, and dMRC Laboratory of Molecular Biology, Cambridge (UK)
The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.
Request reprints from Dr. P.B. Singh, Cell Determination Group, Department of Development and Signalling, AFRC Babraham Institute, Babraham, Cambridge CB2 4AT (UK). Present address of K.A. W.: The Faculty of Pharmacy, 19 Russell Street, University of Toronto, Toronto, Ontario M5S 2S2 (Canada).
Received: 11 August 1993
Published online: May 15, 2008
Number of Print Pages : 5
Cytogenetic and Genome Research
Vol. 66, No. 2, Year 1994 (Cover Date: 1994)
Journal Editor: Schmid M. (Würzburg)
ISSN: 1424–8581 (Print), eISSN: 1424–859X (Online)
For additional information: http://www.karger.com/CGR