Fragments of the spacer region of genes for rat 5S ribosomal RNA (rDNA), which are tandemly repeated, were amplified by PCR with primers specific to the two ends of the coding region for 5S rRNA. Two amplified fragments of ∼1.6 kb were subcloned and sequenced. The spacer sequences showed a high degree of sequence identity to each other (99.2%) but substantial divergence from those of analogous mouse clones. The homologous regions in the mouse clones were interrupted by the duplication or deletion of small segments of DNA. A 12-mer, 5-GGCTCTTGGGGC-3’, thought to be responsible for efficient transcription, was located from position -33 to position -22 in the rat 5S clones. The genes were mapped by fluorescence in situ hybridization with cloned fragments of rat 5S rDNA as probe. The genes were localized exclusively in a single telomeric region of chromosome 19.
Request reprints from Dr. Hitoshi Suzuki, Division of Molecular Genetics, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105 (Japan); telephone: 03-3433-1111 (ext 2372); fax: 03-33435-1922: e-mail: htsuzuki@Tansei.cc.u-tokyo.ac.jp.
Received: 21 February 1995
Published online: May 16, 2008
Number of Print Pages : 4
Cytogenetic and Genome Research
Vol. 72, No. 1, Year 1996 (Cover Date: 1996)
Journal Editor: Schmid M. (Würzburg)
ISSN: 1424–8581 (Print), eISSN: 1424–859X (Online)
For additional information: http://www.karger.com/CGR
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