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Vol. 76, No. 3-4, 1997
Issue release date: 1997
Section title: Gene Mapping, Cloning, and Sequencing
Cytogenet Cell Genet 1997;76:139–143
(DOI:10.1159/000134532)

Genomic organization and mapping of the human HEP-COP gene (COPA) to 1q

Quek H.H. · Chow V.T.K.
Human Genome Laboratory, Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore (Republic of Singapore)

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Article / Publication Details

First-Page Preview
Abstract of Gene Mapping, Cloning, and Sequencing

Accepted: 8/9/1996
Published online: 5/20/2008

Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COP). Seven COP subunits have been recognized, and represent components of a complex known as coatomer. We have previously isolated the cDNA of the human homolog of α-COP, designated HEP-COP and given the official gene symbol COPA. Here we report the genomic organization of COPA, which contains 33 exons ranging in size from 67 to 611 bp. Mapped by PCR and cycle sequencing, all the exon-intron junctions conformed with the GT-AG rule, the 32 introns ranging from about 80 bp to 4 kbp, with the genomic DNA of COPA estimated to span ∼ 37 kb. Southern blot analysis of genomic DNAs of nine eukaryotic species, from human to yeast, revealed identical signals totaling 36 kb each for man and monkey only. Using 5’ RACE and primer extension analysis, the putative transcriptional start site was localized to 466 nucleotides upstream of the translation initiation codon. Comprising a 126-nucleotide 5’ untranscribed genomic sequence and a 466-nucleotide 5’ noncoding cDNA sequence, the 592-nucleotide 5’ CpG island lacked TATA and CAAT boxes but displayed a high G+C content, was enriched for CpG dinucleotides, and contained a potential Spl-binding site, i.e., features compatible with a housekeeping gene. COPA was mapped by fluorescence in situ hybridization to chromosome region 1q23→q25.


  

Author Contacts

Request reprints from Dr. Vincent T.K. Chow, Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore 119260 (Republic of Singapore); telephone: 65-772-6200; fax: 65-776-6872; e-mail: micctk@nus.sg.

  

Article Information

Received: 9 August 1996
Published online: May 20, 2008
Number of Print Pages : 5

  

Publication Details

Cytogenetic and Genome Research

Vol. 76, No. 3-4, Year 1997 (Cover Date: 1997)

Journal Editor: Schmid M. (Würzburg)
ISSN: 1424–8581 (Print), eISSN: 1424–859X (Online)

For additional information: http://www.karger.com/CGR


Article / Publication Details

First-Page Preview
Abstract of Gene Mapping, Cloning, and Sequencing

Accepted: 8/9/1996
Published online: 5/20/2008

Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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