Automated Electron-Capture Gas Chromatographic Analysis of Flunitrazepam in PlasmaGreenblatt D.J. · Ochs H.R. · Locniskar A. · Lauven P.M.
Division of Clinical Pharmacology, Departments of Psychiatry and Medicine, Tufts-New England Medical Center, Boston, Mass., USA; Departments of Medicine and Anesthesia, Medizinische Universitätskliniken, Bonn-Venusberg, FRG
Nanogram quantities of flunitrazepam and its major metabolite, desmethylflunitrazepam, can be reliably quantitated in human plasma using electron-capture gas-liquid chromatography. After addition of methylnitrazepam as the internal standard, plasma samples are extracted with an organic solvent (benzene:isoamyl alcohol). The organic extract is separated, evaporated to dryness, reconstituted, and injected onto the chromatograph using an automatic sampling system which allows up to 100 analyses/24 h. The sensitivity limits are 0.5 ng/ml for flunitrazepam and 1–2 ng/ml for desmethylflunitrazepam. Use of the method is illustrated in a study of the pharmacokinetic properties of flunitrazepam following a single 1.0-mg intravenous dose.
© 1982 S. Karger AG, Basel
David J. Greenblatt, MD, Division of Clinical Pharmacology, Box 1007, New England Medical Center Hospital, 171 Harrison Avenue, Boston, MA 02111 (USA)
Received: April 11, 1981
Accepted: May 5, 1981
Published online: May 30, 2008
Number of Print Pages : 6
Pharmacology (International Journal of Experimental and Clinical Pharmacology)
Vol. 24, No. 2, Year 1982 (Cover Date: 1982)
Journal Editor: Donnerer J. (Graz), Billingsley M.L. (Hershey, Pa.), Maeyama K. (Matsuyama)
ISSN: 0031–7012 (Print), eISSN: 1423–0313 (Online)
For additional information: http://www.karger.com/PHA