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Table of Contents
Vol. 24, No. 2, 1982
Issue release date: 1982
Section title: Original Paper
Pharmacology 1982;24:82–87
(DOI:10.1159/000137580)

Automated Electron-Capture Gas Chromatographic Analysis of Flunitrazepam in Plasma

Greenblatt D.J. · Ochs H.R. · Locniskar A. · Lauven P.M.
Division of Clinical Pharmacology, Departments of Psychiatry and Medicine, Tufts-New England Medical Center, Boston, Mass., USA; Departments of Medicine and Anesthesia, Medizinische Universitätskliniken, Bonn-Venusberg, FRG

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 4/11/1981
Accepted: 5/5/1981
Published online: 5/30/2008
Issue release date: 1982

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA

Abstract

Nanogram quantities of flunitrazepam and its major metabolite, desmethylflunitrazepam, can be reliably quantitated in human plasma using electron-capture gas-liquid chromatography. After addition of methylnitrazepam as the internal standard, plasma samples are extracted with an organic solvent (benzene:isoamyl alcohol). The organic extract is separated, evaporated to dryness, reconstituted, and injected onto the chromatograph using an automatic sampling system which allows up to 100 analyses/24 h. The sensitivity limits are 0.5 ng/ml for flunitrazepam and 1–2 ng/ml for desmethylflunitrazepam. Use of the method is illustrated in a study of the pharmacokinetic properties of flunitrazepam following a single 1.0-mg intravenous dose.

© 1982 S. Karger AG, Basel


  

Author Contacts

David J. Greenblatt, MD, Division of Clinical Pharmacology, Box 1007, New England Medical Center Hospital, 171 Harrison Avenue, Boston, MA 02111 (USA)

  

Article Information

Received: April 11, 1981
Accepted: May 5, 1981
Published online: May 30, 2008
Number of Print Pages : 6

  

Publication Details

Pharmacology (International Journal of Experimental and Clinical Pharmacology)

Vol. 24, No. 2, Year 1982 (Cover Date: 1982)

Journal Editor: Donnerer J. (Graz), Billingsley M.L. (Hershey, Pa.), Maeyama K. (Matsuyama)
ISSN: 0031–7012 (Print), eISSN: 1423–0313 (Online)

For additional information: http://www.karger.com/PHA


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 4/11/1981
Accepted: 5/5/1981
Published online: 5/30/2008
Issue release date: 1982

Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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