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Table of Contents
Vol. 16, No. 3-4, 2009
Issue release date: February 2009
Section title: Research Article
J Mol Microbiol Biotechnol 2009;16:198–207
(DOI:10.1159/000142530)

Activity of Exporters of Escherichia coli in Corynebacterium glutamicum, and Their Use to Increase L-Threonine Production

Diesveld R.a · Tietze N.a · Fürst O.a · Reth A.b · Bathe B.b · Sahm H.a · Eggeling L.a
aInstitute of Biotechnology, Forschungszentrum Jülich, Jülich, and bFeed Additives, Evonik Degussa GmbH, Künsebeck, Germany

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Article / Publication Details

First-Page Preview
Abstract of Research Article

Published online: July 01, 2008
Issue release date: February 2009

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 3

ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

For additional information: http://www.karger.com/MMB

Abstract

L-Threonine is an important biotechnological product and Corynebacterium glutamicum is able to synthesize and accumulate this amino acid to high intracellular levels. We here use four exporters of Escherichia coli and show that three of them operate in C. glutamicum, with RhtA and RhtC being the most effective. Whereas RhtA was unspecific, resulting in L-homoserine together with L-threonine excretion, this was not the case with RhtC. Expression of rhtC reduced the intracellular L-threonine concentration from 140 to 11 mM and resulted in maximal excretion rates of 11.2 nmol min–1 mg–1 as compared to 2.3 nmol min–1 mg–1 obtained without rhtC expression. In combination with an ilvA mutation generated and introduced into the chromosome, an accumulation of up to 54 mML-threonine was achieved as compared to 21 mM obtained with the ancestor strain. This shows that expression of rhtC is the pivotal point for industrial relevant L-threonine production with C. glutamicum, and might encourage in general the use of heterologous exporters in the field of white biotechnology to make full use of biosynthesis pathways.

© 2008 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Research Article

Published online: July 01, 2008
Issue release date: February 2009

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 3

ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)

For additional information: http://www.karger.com/MMB


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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