Journal Mobile Options
Table of Contents
Vol. 154, No. 2, 1995
Issue release date: 1995
Acta Anatomica 1995;154:111–119
Original Paper

A Subset of SBA Lectin-Binding Proteins Isolated from Myocardial-Conditioned Media Transforms Cardiac Endothelium into Mesenchyme

Sinning A.R.a · Hewitt C.C.a · Markwald R.R.b
aDepartment of Anatomy, University of Mississippi Medical Center, Jackson, Miss., bDepartment of Anatomy and Cell Biology, Medical University of South Carolina, Charleston, S.C., USA


It has been shown that the inductively active proteins for cardiac mesenchyme formation are localized to a particulate form of extracellular matrix that resembles adheron-like complexes. These complexes are extractable from the embryonic heart using EDTA and can be visualized with the lectin SBA (Glycine max). In addition, the growth medium obtained from embryonic myocardial cell cultures has also been shown to support mesenchyme formation. However, except for the identification of EDTA extract and conditioned media, all previous experiments analyzing this system have relied on negative type results (i.e. the loss of biological activity) to show a relationship between the particulate matrix and the transformation process. We report here that SBA affinity chromatography can be used to partially purify a subset of proteins from myocardial conditioned medium which elicits the transformation of endothelial cells into mesenchyme. In addition, a polyclonal antibody made against this subset of proteins is specific for the in situ particulate matrix and recognizes several proteins in conditioned medium and EDTA extracts. This antibody is also specific for matrix particulates in other aeas of the embryo that undergo an epithelial/mesenchymal interaction. These results provide the most direct evidence to date that conditioned medium is equivalent to the hypothesized inductively active particulate matrix. In addition, the data provides evidence that conditioned medium can be used to identify the functional role of the components of the particulate matrix in mesenchyme formation.

 goto top of outline Author Contacts

Dr. Allan R. Sinning, Department of Anatomy, University of Mississippi Medical Center, 2500 N. State Street, Jackson, MS 39216 (USA)

 goto top of outline Article Information

Received: March 17, 1995
Accepted: October 16, 1995
Published online: July 17, 2008
Number of Print Pages : 9

 goto top of outline Publication Details

Cells Tissues Organs (in vivo, in vitro)

Vol. 154, No. 2, Year 1995 (Cover Date: 1995)

Journal Editor: Denker H.-W. (Essen), English A.W. (Atlanta, Ga.)
ISSN: 1422–6405 (Print), eISSN: 1422–6421 (Online)

For additional information:

Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.