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Vol. 29, No. 3, 2008
Issue release date: September 2008
Section title: Research Article
Tumor Biol 2008;29:195–203
(DOI:10.1159/000148187)

Elevated Expression of UBE2T in Lung Cancer Tumors and Cell Lines

Hao J. · Xu A. · Xie X. · Hao J. · Tian T. · Gao S. · Xiao X. · He D.
aKey Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, and bDepartment of Chest Surgery, Cancer Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing, China

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Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: 4/18/2008
Accepted: 5/30/2008
Published online: 7/31/2008

Number of Print Pages: 9
Number of Figures: 4
Number of Tables: 2

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: http://www.karger.com/TBI

Abstract

The aim of this study was to investigate the association between UBE2T, a member of the ubiquitin-conjugating E2 family, and lung cancer, which has never been reported to date. Therefore, the expression of UBE2T mRNA was examined in normal human tissues and 8 lung cancer cell lines. Subsequently, UBE2T expression was analyzed in 41 lung cancer tissues by PCR and Western blots, as well as in 103 lung cancer specimens by immunohistochemistry. To further elucidate the possible functional role of UBE2T, the protein was overexpressed in NIH3T3 cells. UBE2T mRNA was highly expressed in all lung cancer cell lines examined, while it could not be detected in normal lung tissue. UBE2T was detected in 75.6% of primary lung cancer tissue samples (n = 41) at mRNA level and in 60.9% at protein level. In addition, positive UBE2T staining was observed in 61% of lung cancer specimens (n = 103), particularly in all immunohistochemically stained small cell carcinoma tissues. In normal lung tissue, only weak staining was observed in the basal cells of bronchial epithelium. Overexpression of UBE2T in NIH3T3 cells significantly promoted colony formation in soft agar medium (p < 0.001). In conclusion, UBE2T was significantly upregulated in lung cancer tissue and cell lines, suggesting involvement of UBE2T in the malignant cell phenotype.


  

Author Contacts

Dacheng He
Department of Life Sciences, Beijing Normal University
Beijing 100875 (PR China)
Tel. +86 10 5880 8439, Fax +86 10 5880 2007
E-Mail dhe@bnu.edu.cn; xyxiao@bnu.edu.cn

  

Article Information

Received: April 18, 2008
Accepted: May 30, 2008
Published online: July 31, 2008
Number of Print Pages : 9
Number of Figures : 4, Number of Tables : 2, Number of References : 19

  

Publication Details

Tumor Biology (Tumor Markers, Tumor Targeting and Translational Cancer Research)

Vol. 29, No. 3, Year 2008 (Cover Date: September 2008)

Journal Editor: Stigbrand T. (Umeå), Rye P.D. (Oslo)
ISSN: 1010–4283 (Print), eISSN: 1423–0380 (Online)

For additional information: http://www.karger.com/TBI


Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: 4/18/2008
Accepted: 5/30/2008
Published online: 7/31/2008

Number of Print Pages: 9
Number of Figures: 4
Number of Tables: 2

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: http://www.karger.com/TBI


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