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Vol. 82, No. 1-2, 1998
Issue release date: 1998
Section title: Paper
Cytogenet Cell Genet 82:115–119 (1998)
(DOI:10.1159/000015082)

lntron based radiation hybrid mapping of 15 complex I genes of the human electron transport chain

Emahazion T. · Beskow A. · Gyllensten U. · Brookes A.J.
Medical Genetics Unit, Department of Genetics and Pathology, Biomedical Center, Uppsala University, Uppsala (Sweden)

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 10/5/1998

Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 4

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

Abstract.

At least 34 complex I subunits of the electron transport chain are encoded by the nuclear genome, but only 14 of these have been mapped in the human. To rapidly map additional subunits, we have performed a combination of database mining and direct “wet” experimentation to identify intron and/or 5′ upstream genomic DNA regions for 16 complex I genes. Wet experimentation was applied to 5 genes, and involved direct PCR amplification of introns by inter-exon PCR or splinkerette based PCR walking. Database mining was applied to 11 genes, and entailed the identification of incompletely spliced mRNAs and genomic CpG island clone sequences. This data was in files that carried no documentary description of the non-exon regions. Non-exon sequences were thus derived for 15 complex I genes and used to design functional gene specific PCR assays. Radiation hybrid mapping of these PCRs located 15 complex I genes to chromosomes l, 4, 5 (2 genes), 7 (2 genes), 8, 9 (2 genes), 11, 14, 16 (2 genes), 18, and 19.


  

Author Contacts

Request reprints from Dr. Anthony J. Brookes, Medical Genetics Unit,Department of Genetics and Pathology, Biomedical Center,Uppsala University, Box 589, S-751 23 Uppsala (Sweden);telephone: +46 (18) 471 41 51; fax: +46 (18) 526 849;e-mail: tony.brookes@medgen.uu.se

  

Article Information

Received: Received 24 April 1998;
manuscript accepted 10 June 1998.
Number of Print Pages : 5
Number of Figures : 0, Number of Tables : 4, Number of References : 36

  

Publication Details

Cytogenetics and Cell Genetics
Founded 1962 as Cytogenetics by H.P. Klinger

Vol. 82, No. 1-2, Year 1998 (Cover Date: 1998)

Journal Editor: H.P. Klinger, New York, USA
ISSN: 0301–0171 (print), 1422–9816 (Online)

For additional information: http://www.karger.com/journals/ccg


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 10/5/1998

Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 4

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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