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Vol. 189, No. 1-4, 2009
Issue release date: December 2008
Section title: Paper
Cells Tissues Organs 2009;189:44–50
(DOI:10.1159/000151430)

Role of Phosphate Groups in Inhibition of Calcium Oxalate Crystal Growth by Osteopontin

Hunter G.K.a · Grohe B.a · Jeffrey S.a · O’Young J.a · Sørensen E.S.b · Goldberg H.A.a
aSchool of Dentistry and CIHR Group in Skeletal Development and Remodeling, University of Western Ontario, London, Ont., Canada; bDepartment of Molecular Biology and Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, Aarhus, Denmark

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 8/15/2008
Issue release date: December 2008

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

Osteopontin (OPN) inhibits the growth of calcium oxalate monohydrate (COM) and other crystal phases in a phosphorylation-dependent manner. In the present study, the role of OPN phosphate groups in adsorption to, incorporation into and inhibition of COM crystals was studied by comparing OPN isoforms differing in phosphorylation. OPN isoforms purified from rat bone (bOPN), which contains 10 phosphates, and cow milk (mOPN), which contains 25 phosphates, were compared with rat recombinant OPN (rOPN), which is not phosphorylated. Using fluorescence-labeled proteins and confocal microscopy, we show that mOPN and rOPN, like bOPN, adsorb preferentially to the edges between {100} and {121} faces of preformed COM crystals, and to a lesser extent to the {100} and {121} faces. Using scanning electron microscopy, we show that growth of COM in the presence of bOPN or mOPN results in a ‘dumbbell’ morphology, whereas crystals grown with rOPN are only slightly affected. COM crystals grown in the presence of low concentrations of fluorescence-labeled bOPN incorporate the protein into the crystal lattice. In crystals imaged in the {010} plane, incorporation of bOPN results in a cross-shaped pattern of fluorescence, consistent with preferential adsorption to {100}/{121} edges throughout the growth process.

© 2008 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 8/15/2008
Issue release date: December 2008

Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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