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Vol. 189, No. 1-4, 2009
Issue release date: December 2008
Section title: Paper
Cells Tissues Organs 2009;189:261–267
(DOI:10.1159/000151743)

Enamel Tissue Engineering Using Subcultured Enamel Organ Epithelial Cells in Combination with Dental Pulp Cells

Honda M.J.a, b · Shinmura Y.c · Shinohara Y.b
aDepartment of Anatomy, Nihon University School of Dentistry, bDivision of Stem Cell Engineering, and cDepartment of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 8/27/2008
Issue release date: December 2008

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

We describe a strategy for the in vitro engineering of enamel tissue using a novel technique for culturing enamel organ epithelial (EOE) cells isolated from the enamel organ using 3T3-J2 cells as a feeder layer. These subcultured EOE cells retain the capacity to produce enamel structures over a period of extended culture. In brief, enamel organs from 6-month-old porcine third molars were dissociated into single cells and subcultured on 3T3-J2 feeder cell layers. These subcultured EOE cells were then seeded onto a collagen sponge in combination with primary dental pulp cells isolated at an early stage of crown formation, and these constructs were transplanted into athymic rats. After 4 weeks, complex enamel-dentin structures were detected in the implants. These results show that our culture technique maintained ameloblast lineage cells that were able to produce enamel in vivo. This novel subculture technique provides an important tool for tooth tissue engineering.

© 2008 S. Karger AG, Basel


  

Author Contacts

Dr. Masaki J. Honda
Nihon University School of Dentistry, Department of Anatomy
1-8-13 Kanda-Surugadai Chiyoda-ku
Tokyo 101-8310 (Japan)
Tel. +81 3 3219 8121, Fax +81 3 3219 8319, E-Mail honda-ms@dent.nihon-u.ac.jp

  

Article Information

Published online: August 27, 2008
Number of Print Pages : 7
Number of Figures : 3, Number of Tables : 1, Number of References : 25

  

Publication Details

Cells Tissues Organs (in vivo, in vitro)

Vol. 189, No. 1-4, Year 2009 (Cover Date: December 2008)

Journal Editor: Denker H.-W. (Essen), English A.W. (Atlanta, Ga.)
ISSN: 1422-6405 (Print), eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 8/27/2008
Issue release date: December 2008

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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