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Vol. 83, No. 3-4, 1998
Issue release date: 1998
Section title: Paper
Cytogenet Cell Genet 83:214–217 (1998)
(DOI:10.1159/000015181)

Molecular cloning, expression, and chromosomal localization of a ubiquitously expressed human 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase gene (PFKFB3)

Manzano A. · Rosa J.L. · Ventura F. · Pérez J.X. · Nadal M. · Estivill X. · Ambrosio S. · Gil J. · Bartrons R.
aUnitat de Bioquímica, Campus de Bellvitge, Universitat de Barcelona, and bCentre de Genètica Mèdica i Molecular, Institut de Recerca Oncològica, L’Hospitalet, Barcelona (Spain)

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 3/3/1999
Issue release date: 1998

Number of Print Pages: 4
Number of Figures: 4
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

Abstract.

We report the identification of a human 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase gene (PFKFB3) isolated from a human fetal brain cDNA library. The gene was localized to 10p15→p14 by fluorescence in situ hybridization. The entire cDNA (4,322 bp) codes for a polypeptide of 520 amino acid residues (molecular weight, 59.571 kDa). Structural analysis showed the presence of a kinase domain located at the amino terminus and a bisphosphatase domain at the carboxy terminus, characteristic of previously described 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase isozymes. In addition, a phosphorylation site for cAMP-dependent protein kinase was found at the carboxy terminus. Northern blot analysis showed the presence of a unique 4.8-kb mRNA expressed in the different tissues studied. In mammalian COS-1 cells, this cDNA drives the expression of an active isozyme. Taken together, these results identify the presence of a gene coding for a human 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase isozyme which is ubiquitously expressed.


  

Author Contacts

Request reprints from Dr. Ramon Bartrons, Unitat de Bioquímica, Campus de Bellvitge, Universitat de Barcelona, C/Feixa Llarga S/N, 08907 L’Hospitalet, Barcelona (Spain); telephone: 34-402-42-52; fax: 34-402-42-68; e-mail: bartrons@bellvitge.bvg.ub.es.

  

Article Information

Supported by the Fondo de Investigaciones Sanitarias (FIS, 95/0286) of Spain and the Generalitat de Catalunya (1997SGR00074). A.M. and J.L.R received research fellowships from the Ministerio de Educación y Ciencia; X.P. had a research fellowship from FIS.

Received: Received 29 June 1998;
revision accepted 16 November 1998.
Number of Print Pages : 4
Number of Figures : 4, Number of Tables : 0, Number of References : 32

  

Publication Details

Cytogenetics and Cell Genetics
Founded 1962 as Cytogenetics by H.P. Klinger

Vol. 83, No. 3-4, Year 1998 (Cover Date: 1998)

Journal Editor: H.P. Klinger, New York, USA
ISSN: 0301–0171 (print), 1422–9816 (Online)

For additional information: http://www.karger.com/journals/ccg


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 3/3/1999
Issue release date: 1998

Number of Print Pages: 4
Number of Figures: 4
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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