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Table of Contents
Vol. 87, No. 3-4, 1999
Issue release date: 1999
Section title: Paper
Cytogenet Cell Genet 87:278–281 (1999)
(DOI:10.1159/000015449)

Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome

Griffin D.K.a,b · Haberman F.b,c · Masabanda J.a · O’Brien P.b · Bagga M.b · Sazanov A.d · Smith J.e · Burt D.W.e · Ferguson-Smith M.b · Wienberg J.b,e
aDepartment of Biological Sciences, Brunel University, Uxbridge, Middlesex (UK); bDepartment of Pathology, University of Cambridge, Cambridge (UK); cInstitut für Anthropologie und Humangenetik, Ludwig-Maximilians Universität, München (Germany); dThe Roslin Institute, Roslin, Midlothian, Scotland (UK); and eLehrstuhl für Tierzucht, Technische Universität München, Freising-Weihenstephan (Germany)

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: February 18, 2000
Issue release date: 1999

Number of Print Pages: 4
Number of Figures: 4
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

Abstract.

Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.   

© 2000 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: February 18, 2000
Issue release date: 1999

Number of Print Pages: 4
Number of Figures: 4
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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