The mouse genes encoding β-galactoside α2,3-sialyltransferases—Siat4 (ST3Gal I), Siat5 (ST3Gal II), Siat3 (ST3Gal III), and Siat4c (ST3Gal IV)—were isolated and characterized. Siat4 and Siat5 comprise 8.4 and 14 kb, respectively, and are composed of six exons each. The genomic structures of the two genes were similar. Siat3 and Siat4c comprise over 100 and 9.7 kb, respectively, and are composed of 12 and 10 exons, respectively. Although the genomic sizes of these genes differ, some of their exon structures are significantly similar. These results suggest that the gene pair Siat4 and Siat5 arose from a common ancestral gene, as did the two genes Siat3 and Siat4c.
Request reprints from Dr. Shuichi Tsuji, Molecular Glycobiology, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198 (Japan); telephone: +81-463-85-5301; fax: +81-463-85-5301, e-mail: email@example.com.
Supported by the Ministry of Education of Japan (Grants-in-Aid Nos. 10152263, 10178104, and 10178105 for Scientific Research on Priority Areas and Grant-in-Aid No. B11480184 for Scientific Research). The nucleotide sequences reported in this paper have been submitted to the GenBank/EBI Data Bank under accession numbers X73523 (Siat4 [mouse ST3Gal I]), X76989 (Siat5 [mouse ST3Gal II]), X84234 (Siat3 [mouse ST3Gal III]), and X95809 (Siat4c [mouse ST3Gal IV]). The nomenclature for gangliosides follows the system of Svennerholm (1964). The abbreviated nomenclature for cloned sialyltransferases follows the system of Tsuji et al. (1996): Gal = galactose; GalNAc = N-acetylgalactosamine; Sia = sialic acid. Shou Takashima’s present address is Neural Regeneration, Brain Science Institute, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198 (Japan).
Received: Received 18 November 1999;
revision accepted 20 March 2000.
Number of Print Pages : 6
Number of Figures : 2, Number of Tables : 4, Number of References : 32
Cytogenetics and Cell Genetics
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Vol. 89, No. 1-2, Year 2000 (Cover Date: 2000)
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Published online: 6/26/2000
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Number of Print Pages: 6
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