The Localization and Secretion of Type IV Collagen in Synovial Capillaries by Immunohistochemistry Using a Monoclonal Antibody against Human Type IV CollagenMatsubara T. · Trüeb B. · Fehr K. · Rüttner J.R. · Odermatt B.F.
Department of aPathology and bRheumatology, University Hospital, Zürich, Switzerland
The localization and the secretion of type IV collagen in synovial capillaries have been investigated by detecting the antigenic determinant of the major triple helix of human type IV collagen. Type IV collagen was indicated to be localized mainly in the lamina densa of basement membranes (BM) and to be secreted by both endothelial cells and pericytes. The pericytes secreted this collagen to both surfaces facing endothelial cells and the interstitial connective tissue. On the contrary, the direction of type IV collagen secretion by the endothelial cells was strictly confined to one side, namely towards the surface facing the BM. The absence of the antigenic determinant in rough endoplasmic reticulum and Golgi apparatus of the endothelial cells and pericytes indicated that the major triple helix of type IV collagen is mainly formed in the secretory vesicles after budding from the Golgi apparatus.
Dr. Tsukasa Matsubara, Department of Orthopaedic Surgery, Kobe University School of Medicine, 7 chome, Kusunoki-cho, Chuo-ku, Kobe (Japan)
Received: May 30, 1983
Accepted: June 23, 1983
Published online: October 07, 2008
Number of Print Pages : 11
Pathobiology (Exploring the basis of disease)
Vol. 52, No. 3, Year 1984 (Cover Date: 1984)
Journal Editor: Borisch B. (Geneva), Yasui W. (Hiroshima)
ISSN: 1015–2008 (Print), eISSN: 1423–0291 (Online)
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