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Vol. 58, No. 4, 1990
Issue release date: 1990
Section title: Alpha-MSH Immunoreactivity
Pathobiology 1990;58:193–197
(DOI:10.1159/000163583)

Alpha-Melanocyte-Stimulating Hormone Immunoreactivity in Melanoma Cells

Lunec J. · Pieron C. · Sherbet G.V. · Thody A.J.
Cancer Research Unit, Medical School, University of Newcastle-upon-Tyne, UK

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Article / Publication Details

First-Page Preview
Abstract of Alpha-MSH Immunoreactivity

Received: 1/5/1990
Accepted: 3/30/1990
Published online: 10/7/2008

Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: http://www.karger.com/PAT

Abstract

Using a radioimmunoassay specific for α-melanocyte-stimulating hormone (α-MSH), significant levels of immunoreactivity were detected in a range of murine and human melanoma cell lines, including a series of ras-transfected melanocytes. The levels found in the melanoma cell lines tested varied, and overall were higher than in non-melanoma cell lines assayed for comparison. Furthermore the highest levels of immunoreactivity measured tended to be in the least differentiated and most metastatic melanoma lines. High performance liquid chromatography showed a peak of immunoreactivity which co-migrated with a desacetyl α-MSH standard. Additional unidentified components of immunoreactivity were found, including a high molecular weight form revealed by Sephadex-G50 gel exclusion. These may represent bound α-MSH or fragments of the proopiomelanocortin precursor having in common the C-terminus epitope recognised by the antibody. In view of the known effects of α-MSH on anchorage independent growth and metastasis of melanoma cells, our findings raise the possibility that MSH peptides may have an autocrine role in the growth and progression of melanoma. However, further characterisation of the immunore-active species is required to determine whether these represent biologically active forms.


  

Author Contacts

Dr. J. Lunec, Cancer Research Unit, Medical School, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne (UK)

  

Article Information

Received: January 5, 1990
Accepted in revised form: March 30, 1990
Published online: October 07, 2008
Number of Print Pages : 5

  

Publication Details

Pathobiology (Exploring the basis of disease)

Vol. 58, No. 4, Year 1990 (Cover Date: 1990)

Journal Editor: Borisch B. (Geneva), Yasui W. (Hiroshima)
ISSN: 1015–2008 (Print), eISSN: 1423–0291 (Online)

For additional information: http://www.karger.com/PAT


Article / Publication Details

First-Page Preview
Abstract of Alpha-MSH Immunoreactivity

Received: 1/5/1990
Accepted: 3/30/1990
Published online: 10/7/2008

Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: http://www.karger.com/PAT


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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