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Vol. 65, No. 6, 1997
Issue release date: 1997
Section title: Original Paper
Pathobiology 1997;65:293–300
(DOI:10.1159/000164141)

Expression of Five Selected Human Mismatch Repair Genes Simultaneously Detected in Normal and Cancer Cell Lines by a Nonradioactive Multiplex Reverse Transcription-Polymerase Chain Reaction

Wei Q.a · Guan Y.a · Cheng L.a · Radinsky R.b · Bar-Eli M.b · Tsan R.b · Li L.c · Legerski R.J.c
Departments of aEpidemiology, bCell Biology and cMolecular Genetics, The University of Texas M.D. Anderson Cancer Center, Houston, Tex., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 8/14/1997
Accepted: 9/3/1997
Published online: 10/8/2008
Issue release date: 1997

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: http://www.karger.com/PAT

Abstract

Abnormalities in at least 1 of 5 mismatch repair (MMR) genes (hMSH2, hMLH1, hPMS1, hPMS2 and GTBP/hMSH6) are found in hereditary nonpolyposis colon cancer and sporadic colon cancers. We used a single-reaction multiplex reverse transcription (RT)-polymerase chain reaction (PCR), with the β-actin gene as an internal control, to simultaneously evaluate expression of these 5 known human MMR genes in normal and tumor cell lines with known or uncharacterized mutations in MMR genes. The relative quantitation of the transcripts is demonstrated by controlling the number of PCR cycles and titrating cDNA with a dose-curve. The 13 normal cell lines tested were derived from normal lymphocytes, skin, thymus, breast, lung, colon, liver and kidney. The 26 cancer cell lines were derived from melanoma and cancers of the brain, breast, lung, colon, pancreas and prostate. All 5 MMR genes were ubiquitously expressed in all normal cell lines tested, suggesting their housekeeping roles. Aberrant MMR gene expression was only observed in the colon cancer cell lines. Two previously uncharacterized colon cancer cell lines did not express hMLH1. These data suggest that this nonradioactive multiplex RT-PCR assay for MMR gene expression may be useful for fast screening for genetic alterations that may affect gene expression and so may aid molecular analysis of MMR-related colon cancer.

© 1997 S. Karger AG, Basel


  

Author Contacts

Qingyi Wei, Department of Epidemiology, University of Texas M.D. Anderson Cancer Center, Box 189, 1515 Holcombe Blvd., Houston, TX 77030 (USA), Tel. +1713 792 3020, Fax +1 713 792 0807, E-Mail qwei@request.mdacc.tmc.edu

  

Article Information

Received: August 14, 1997
Accepted: September 3, 1997
Published online: October 08, 2008
Number of Print Pages : 8

  

Publication Details

Pathobiology (Exploring the basis of disease)

Vol. 65, No. 6, Year 1997 (Cover Date: 1997)

Journal Editor: Borisch B. (Geneva), Yasui W. (Hiroshima)
ISSN: 1015–2008 (Print), eISSN: 1423–0291 (Online)

For additional information: http://www.karger.com/PAT


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 8/14/1997
Accepted: 9/3/1997
Published online: 10/8/2008
Issue release date: 1997

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)

For additional information: http://www.karger.com/PAT


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