Immunohistochemical Analysis of Cell Cycle-Related Molecules in Gastric Carcinoma: Prognostic Significance, Correlation with Clinicopathological Parameters, Proliferation and ApoptosisLee K.-H.a · Lee H.E.f · Cho S.J.a · Cho Y.J.a · Lee H.S.f · Kim J.H.d · Nam S.Y.e · Chang M.S.b · Kim W.H.b · Lee B.L.a
aDepartment of Anatomy, bDepartment of Pathology and cIschemic/Hypoxic Disease Institute, Seoul National University College of Medicine, dDepartment of Pathology, Asan Medical Center, University of Ulsan College of Medicine, eDivision of Radiation Effect Research, Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Seoul, and fDepartment of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea
Dr. Byung Lan Lee
Department of Anatomy, Seoul National University College of Medicine
28 Yeongon-dong, Chongno-gu
Seoul 110-799 (Korea)
Tel. +82 2 740 8206, Fax +82 2 745 9528, E-Mail email@example.com
Do you have an account?
Objective: We aimed to investigate the biological significance of cell cycle regulators in gastric carcinoma. Methods: Immunohistochemistry and TUNEL staining were performed on tissue array slides containing 293 gastric carcinoma specimens. The relationship between the protein expression of each of the cell cycle regulators and prognosis, clinicopathological features, proliferation, or apoptosis was evaluated. Results: The nuclear immunoreactivity for cyclin D1, cyclin E, p21, and p27 was observed in 22, 14, 31 and 27% of cases, respectively. The expression of cyclin D1, p21, or p27 positively correlated with early pTNM stages, tumor cell proliferation (represented by Ki-67 labeling) and good prognosis, whereas it inversely correlated with the lymph node metastasis (p < 0.05). On the other hand, p27 expression inversely correlated with the apoptosis index represented by TUNEL staining (p < 0.001). In addition, the expression of cyclin D1 positively correlated with that of p21 or p27 (p < 0.05). Conclusions: Our results showed that the expression of cyclin D1, p21 and p27, alone or in combination, are early events in gastric tumorigenesis and may serve as a candidate molecular marker for the early gastric carcinoma.
© 2008 S. Karger AG, Basel
Article / Publication Details
Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.