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Vol. 32, No. 6, 1998
Issue release date: November–December 1998
Section title: Original Paper
Caries Res 1998;32:456–462
(DOI:10.1159/000016487)

Analysis of pH–Driven Disruption of Oral Microbial Communities in vitro

Bradshaw D. · Marsh P.D.
Research Division, CAMR, Salisbury, Wiltshire, UK

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 9/18/1998

Number of Print Pages: 7
Number of Figures: 2
Number of Tables: 2

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE

Abstract

Previously, a mixed culture chemostat system was used to demonstrate that the pH generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. The aim of this study was to determine more accurately the microbially generated pH at which such shifts occurred. Nine oral bacteria were grown in three independent chemostats, and pulsed with glucose on 10 consecutive days. In one chemostat, pH control was discontinued for 6 h, and the pH fall was restricted to a minimum value of pH 5.5; the pH fall was arrested in the other two chemostats at either pH 5.0, or at pH 4.5. When the pH was allowed to fall, the numbers and proportions of Streptococcus mutans and Lactobacillus rhamnosus increased; this increase was directly related to the magnitude of the pH fall. Veillonella dispar was the most numerous organism following all glucose pulsing regimes, especially at low pH. The increase in proportions of acidogenic bacteria was accompanied by a fall in the proportions of acid–sensitive species (Fusobacterium nucleatum, Prevotella nigrescens, Streptococcus gordonii and Streptococcus oralis). The counts of these species were relatively stable between pH 5.5 and 4.5, but were markedly reduced when the pH fell below pH 4.5; Neisseria subflava could not persist in the culture at pH 4.5 or below. The data suggest that the disruption of communities associated with glucose metabolism and low pH can be explained in terms of a two–stage process. A fall in pH to a value between pH 5.5 and 4.5 may allow the enrichment of potentially cariogenic species, whilst permitting species associated with health to remain relatively unaffected. A further reduction in pH (


  

Author Contacts

David J. Bradshaw
Research Division, CAMR
Salisbury, Wiltshire SP4 OJG (UK)
Tel. +44 (0)1980 612 732 (direct), +44 (0)1980 612 100 (switchboard),
Fax +44 (0) 1980 612 731, E–Mail david.bradshaw@camr.org.uk

  

Article Information

Received: Received: December 11, 1997
Accepted after revision: March 2, 1998
Number of Print Pages : 7
Number of Figures : 2, Number of Tables : 2, Number of References : 43

  

Publication Details

Caries Research (Journal of the European Organization for Caries Research (ORCA))
Journal founded 1967 by Y. Ericsson; edited 1970–1987 by K.G. König; 1987–1994 by J.M. ten Cate

Vol. 32, No. 6, Year 1998 (Cover Date: November-December 1998)

Journal Editor: J. Tenovuo, Turku
ISSN: 0008–6568 (print), 1421–976X (Online)

For additional information: http://www.karger.com/journals/cre


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 9/18/1998

Number of Print Pages: 7
Number of Figures: 2
Number of Tables: 2

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE


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