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Vol. 33, No. 5, 1999
Issue release date: September–October 1999
Section title: Original Paper
Caries Res 1999;33:393–400
(DOI:10.1159/000016539)

Effect of Apis mellifera Propolis from Two Brazilian Regions on Caries Development in Desalivated Rats

Koo H. · Rosalen P.L. · Cury J.A. · Park Y.K. · Ikegaki M. · Sattler A.
aDepartment of Physiological Sciences, Faculty of Dentistry of Piracicaba, State University of Campinas, Piracicaba, bDepartment of Food Science, College of Food Engineering, State University of Campinas, cDepartment of Entomology, Faculty of Agricultural Science, Federal University of Rio Grande do Sul – FEPAGRO, Porto Alegre, Brazil

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 8/4/1999

Number of Print Pages: 8
Number of Figures: 2
Number of Tables: 5

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE

Abstract

The purpose of the present study was to evaluate the effect of Apis mellifera propolis collected from two regions of Brazil on caries development in desalivated rats. Ethanolic extracts of propolis (EEP) were prepared from crude propolis samples collected in Minas Gerais state (MG), southeastern Brazil, and Rio Grande do Sul state (RS), southern Brazil. The flavonoid composition of EEP was analyzed by high–performance thin–layer chromatography (HPTLC) and reversed–phase high–performance liquid chromatography (HPLC). For the animal study, 30 specific pathogen–free Wistar rats were infected with Streptococcus sobrinus 6715 and surgically desalivated. The rats were randomly divided into three groups which were treated with 80% ethanol (control), EEP from MG and EEP from RS. The animals were placed in a König–Höfer programmed feeder and received 17 meals of diet 2000 daily at hourly intervals. The solutions were applied on the rat molars (25 μl on molars of each quadrant) twice a day, by using graduate syringes. After 3 weeks, the animals were killed by CO2 asphyxiation. For microbial assessment, the left jaw was removed and sonicated in 154 mM NaCl solution. Dental caries was evaluated according to Larson’s modification of Keyes’ system. The HPTLC patterns and HPLC profiles demonstrated that both quality and quantity of flavonoid aglycones of EEP from MG were different compared to EEP from RS. In general, it is apparent that EEP from RS contained the highest concentrations of pinocembrin, chrysin, acacetin and galangin. The group of animals treated with EEP from RS showed the lowest smooth–surface and sulcal caries scores as well as less caries severity in smooth–surface and sulcal lesions, and these data were statistically different when compared with the control group. The group treated with EEP from MG only demonstrated a significant difference in the severity of sulcal lesions when compared to the control group. The percentage of S. sobrinus was lower in the groups treated with EEP, but did not differ statistically from the control group. The results showed that the cariostatic effect of propolis depends on its composition, and consequently the region of collection of propolis samples.


  

Author Contacts

Jaime A. Cury
FOP/UNICAMP
Av. Limeira, 901, Caixa Postal 52
13414–900 Piracicaba, SP (Brazil)
Tel. +55 19 430 5302, Fax +55 19 430 5218, E–Mail jcury@fop.unicamp.br

  

Article Information

Received: Received: May 30, 1998
Accepted after revision: December 16, 1998
Number of Print Pages : 8
Number of Figures : 2, Number of Tables : 5, Number of References : 47

  

Publication Details

Caries Research (Journal of the European Organization for Caries Research (ORCA))
Journal founded 1967 by Y. Ericsson; edited 1970–1987 by K.G. König; 1987–1994 by J.M. ten Cate

Vol. 33, No. 5, Year 1999 (Cover Date: September-October 1999)

Journal Editor: J. Tenovuo, Turku
ISSN: 0008–6568 (print), 1421–976X (Online)

For additional information: http://www.karger.com/journals/cre


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 8/4/1999

Number of Print Pages: 8
Number of Figures: 2
Number of Tables: 5

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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