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Vol. 22, No. 1-2, 2000
Issue release date: January–April (February 2000)
Section title: Paper
Dev Neurosci 2000;22:139–153
(DOI:10.1159/000017435)

Musashi1: An Evolutionally Conserved Marker for CNS Progenitor Cells Including Neural Stem Cells

Kaneko Y. · Sakakibara S. · Imai T. · Suzuki A. · Nakamura Y. · Sawamoto K. · Ogawa Y. · Toyama Y. · Miyata T. · Okano H.
aDivision of Neuroanatomy, Department of Neuroscience, Biomedical Research Center, Osaka University Graduate School of Medicine; bDepartment of Anatomy, Institute of Basic Medical Sciences, University of Tsukuba; cCore Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), and dDepartment of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 2/15/2000

Number of Print Pages: 15
Number of Figures: 5
Number of Tables: 0

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: http://www.karger.com/DNE

Abstract

In situ detection of neural progenitor cells including stem-like cells is essential for studying the basic mechanisms of the generation of cellular diversity in the CNS, upon which therapeutic treatments for CNS injuries, degenerative diseases, and brain tumors may be based. We have generated rat monoclonal antibodies (Mab 14H1 and 14B8) that recognize an RNA-binding protein Musashi1, but not a Musashi1-related protein, Musashi2. The amino acid sequences at the epitope sites of these anti-Musashi1 Mabs are remarkably conserved among the human, mouse, and Xenopus proteins. Spatiotemporal patterns of Musashi1 immunoreactivity in the developing and/or adult CNS tissues of frogs, birds, rodents, and humans indicated that our anti-Musashi1 Mabs reacted with undifferentiated, proliferative cells in the CNS of all the vertebrates tested. Double or triple immunostaining of embryonic mouse brain cells in monolayer cultures demonstrated strong Musashi1 expression in Nestin(+)/RC2(+) cells. The relative number of Musashi1(+)/Nestin(+)/RC2(+) cells increased fivefold when embryonic forebrain cells were cultured to form ‘neurospheres’ in which stem-like cells are known to be enriched through their self-renewing mode of growth. Nestin(+)/RC2(–) cells, which included Tα1-GFP(+) neuronal progenitor cells and GLAST(+) astroglial precursor cells, were also Musashi1(+), as were GFAP(+) astrocytes. Young neurons showed a trace of Musashi1 expression. Cells committed to the oligodendroglial lineage were Musashi(–). Musashi1 was localized to the perikarya of CNS stem-like cells and non-oligodendroglial progenitor cells without shifting to cell processes or endfeet, and is therefore advantageous for identifying each cell and counting cells in situ.


  

Author Contacts

Hideyuki Okano, MD, PhD, Prof.
Department of Neuroanatomy (D12)
Osaka University Graduate School of Medicine
2-2 Yamadaoka, Suita, Osaka 565-0871 (Japan)
Tel. +81 6 6879 3581, Fax +81 6 6879 3589, E-Mail okano@nana.med.osaka-u.ac.jp

  

Article Information

Received: Received: April 30, 1999
Accepted: May 27, 1999
Number of Print Pages : 15
Number of Figures : 5, Number of Tables : 0, Number of References : 59

  

Publication Details

Developmental Neuroscience

Vol. 22, No. 1-2, Year 2000 (Cover Date: January-April (Released February 2000))

Journal Editor: A.T. Campagnoni, Los Angeles, Calif.
ISSN: 0378–5866 (print), 1421–9859 (Online)

For additional information: http://www.karger.com/journals/dne


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 2/15/2000

Number of Print Pages: 15
Number of Figures: 5
Number of Tables: 0

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: http://www.karger.com/DNE


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