Journal Mobile Options
Table of Contents
Vol. 22, No. 1-2, 2000
Issue release date: January–April (February 2000)
Section title: Paper
Dev Neurosci 2000;22:167–176
(DOI:10.1159/000017437)

Promoter-Based Isolation and Fluorescence-Activated Sorting of Mitotic Neuronal Progenitor Cells from the Adult Mammalian Ependymal/Subependymal Zone

Wang S. · Roy N.S. · Benraiss A. · Goldman S.A.
Department of Neurology and Neuroscience, Cornell University Medical College, New York, N.Y., USA

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 9.00
Account: USD 8.00

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Access to all articles of the subscribed year(s) guaranteed for 5 years
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 2/15/2000
Issue release date: January–April (February 2000)

Number of Print Pages: 10
Number of Figures: 8
Number of Tables: 0

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: http://www.karger.com/DNE

Abstract

Neuronal precursor cells are widespread in the subependyma of the forebrain ventricular lining, and may provide a cellular substrate for brain repair. We have previously identified and isolated them from fetal brain, by sorting forebrain cells transfected with plasmid DNA encoding the gene for green fluorescent protein (hGFP), driven by the early neuronal promoter for Tα1 tubulin (P/Tα1). Fetal neuronal precursors were thereby identified and harvested with both a high degree of enrichment, and a virtual abolition of glial contaminants. We have now extended this approach to include the isolation and purification of neuronal progenitors from the adult brain. Dissociates of the lateral ventricular wall, that included the combined ependymal/subependymal zone, were obtained from 3-month-old adult rats. These cells were cultured and transfected with P/Tα1:hGFP plasmid DNA. Two days later, the cells were redissociated, sorted on the basis of Tα1-driven GFP expression, and replated. The majority of these cells expressed the early neuronal proteins Hu and TuJ1/βIII-tubulin upon FACS; within the week thereafter, most matured as morphologically-evident neurons, that coexpressed βIII-tubulin and MAP-2. Fewer than 5% expressed astrocytic markers, compared to over half of the cells in matched samples that were either not sorted, or sorted after transfection with a plasmid bearing the nonfluorescent lacZ gene under the control of P/Tα1 tubulin. Thus, the use of a fluorescent transgene under the control of an early neuron-selective promoter permits the enrichment of neuronal progenitor cells from the adult rat brain, in a form that may allow their heterologous implantation.


  

Author Contacts

Steven A. Goldman, MD, PhD
Department of Neurology and Neuroscience
Cornell University Medical Center/1300 York Ave, Rm E607
New York, NY 10021 (USA)
Tel. +1 212 746 6572, Fax +1 212 746 8972, E-Mail sgoldm@mail.med.cornell.edu

  

Article Information

Received: Received: June 16, 1999
Accepted: June 22, 1999
Number of Print Pages : 10
Number of Figures : 8, Number of Tables : 0, Number of References : 29

  

Publication Details

Developmental Neuroscience

Vol. 22, No. 1-2, Year 2000 (Cover Date: January-April (Released February 2000))

Journal Editor: A.T. Campagnoni, Los Angeles, Calif.
ISSN: 0378–5866 (print), 1421–9859 (Online)

For additional information: http://www.karger.com/journals/dne


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 2/15/2000
Issue release date: January–April (February 2000)

Number of Print Pages: 10
Number of Figures: 8
Number of Tables: 0

ISSN: 0378-5866 (Print)
eISSN: 1421-9859 (Online)

For additional information: http://www.karger.com/DNE


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.