Journal Mobile Options
Table of Contents
Vol. 7, No. 4, 1999
Issue release date: July–August 1999
Section title: Original Paper
Exp Nephrol 1999;7:306–313
(DOI:10.1159/000020618)

Transdifferentiation of Distal but Not Proximal Tubular Epithelial Cells from Human Kidney in Culture

Baer P.C. · Tunn U.W. · Nunez G. · Scherberich J.E. · Geiger H.
aDivision of Nephrology, Department of Internal Medicine IV, J.W. Goethe University, Frankfurt am Main; bDepartment of Urology, Academic Hospital, Offenbach; cDivision of Nephrology, Department of Medicine II, Hospital München-Harlaching, München, Germany

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 38.00
Account: USD 26.50

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Automatic perpetual access to all articles of the subscribed year(s)
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 7/26/1999

Number of Print Pages: 8
Number of Figures: 5
Number of Tables: 1

ISSN: (Print)
eISSN: 1660-2129 (Online)

For additional information: http://www.karger.com/NEE

Abstract

Human renal proximal and distal (thick ascending limb and early distal convoluted tubule) epithelial cells have been isolated according to their specific antigen expression. The cells were well characterized by flow cytometry, enzyme cytochemistry and electron microscopy and cultured for up to 3 months. Cultured tubular cells coexpressed cytokeratin and vimentin as intermediate filament proteins. While primary isolated cells, proximal as well as distal, revealed the phenotypic characteristics of their nephron origin, cultured distal cells showed the tendency to dedifferentiate/transdifferentiate. Distal cells lost their characteristic expression of Tamm-Horsfall glycoprotein and started de novo expression of the proximal marker proteins aminopeptidase M, γ-glutamyl transferase and dipeptidyl peptidase IV. The expression of these antigens by distal cells could be shown by flow-cytometric analysis and fluorescence microscopy. Enzyme activity assays revealed the activity of aminopeptidase M, γ-glutamyl transferase and dipeptidyl peptidase IV, but not of the proximal marker enzyme alkaline phosphatase. This antigenic shift could not be prevented in different culture media, and the original phenotype could not be restored. Cultured cells displayed characteristic hormonal stimulation patterns indicative of their proximal and distal origins, as shown by activation of adenylate cyclase by different peptide hormones. These results indicate that distal tubular cells possibly transdifferentiate to a more proximal phenotype in view of loss of the distal marker enzyme Tamm-Horsfall protein and de novo expression of proximal marker enzymes like dipeptidyl peptidase IV and aminopeptidase M.


  

Author Contacts

Patrick C. Baer
Johann Wolfgang Goethe University
Division of Nephrology, Department of Internal Medicine IV
Theodor-Stern-Kai 7, D–60590 Frankfurt am Main (Germany)
Tel. +49 69 6301 5554, Fax +49 69 6301 4749, E-Mail P.Baer@em.uni-frankfurt.de

  

Article Information

Received: Received: August 21, 1998
Accepted: November 5, 1998
Number of Print Pages : 8
Number of Figures : 5, Number of Tables : 1, Number of References : 32

  

Publication Details

Experimental Nephrology (European Journal of Renal Research)

Vol. 7, No. 4, Year 1999 (Cover Date: July-August 1999)

Journal Editor: L.G. Fine, London
ISSN: 1018–7782 (print), 1421–9956 (Online)

For additional information: http://www.karger.com/journals/exn


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 7/26/1999

Number of Print Pages: 8
Number of Figures: 5
Number of Tables: 1

ISSN: (Print)
eISSN: 1660-2129 (Online)

For additional information: http://www.karger.com/NEE


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.