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Vol. 71, No. 3, 1984
Issue release date: 1984
Section title: Original Paper
Acta Haematol 1984;71:189–197
(DOI:10.1159/000206583)

CFU-GM Colony-Enhancing Activity in Sera of Dogs under Acute and Chronic Gamma-Irradiation Regimens

Kaspar L.V. · Seed T.M.
Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Ill., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 5/10/1982
Accepted: 7/19/1983
Published online: 2/25/2009
Issue release date: 1984

Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0

ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)

For additional information: http://www.karger.com/AHA

Abstract

Dogs were chronically irradiated (10 R/day; 0–400 R) or acutely irradiated (20 R/min; 0–400 R) with 60Co γ-rays. Sera were collected and assayed for colony-enhancing activity by a double-layer agar cloning technique. When test sera alone were incorporated in the feeder layers, no colony-forming units-granulocyte/macrophage formation occurred; the addition of peripheral blood leukocytes to such test sera-containing feeder layers resulted in formation of colonies, the number of which was directly related to the cumulative radiation dose. Serum-enhancing factor activity indirectly increased colony formation by stimulating feeder layer leukocytes to generate increased levels of colony-stimulating activity. Levels of serum-enhancing activity were higher at similar dose levels following acute gamma irradiation then following chronic irradiation.

© 1984 S. Karger AG, Basel


  

Article Information

Received: May 10, 1982
Accepted: July 19, 1983
Published online: February 25, 2009
Number of Print Pages : 9

  

Publication Details

Acta Haematologica

Vol. 71, No. 3, Year 1984 (Cover Date: 1984)

Journal Editor: Ben-Bassat I. (Qiryat-Ono)
ISSN: 0001-5792 (Print), eISSN: 1421-9662 (Online)

For additional information: http://www.karger.com/AHA


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 5/10/1982
Accepted: 7/19/1983
Published online: 2/25/2009
Issue release date: 1984

Number of Print Pages: 9
Number of Figures: 0
Number of Tables: 0

ISSN: 0001-5792 (Print)
eISSN: 1421-9662 (Online)

For additional information: http://www.karger.com/AHA


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