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Table of Contents
Vol. 96, No. 2, 2009
Issue release date: August 2009
Section title: Original Paper
Neonatology 2009;96:102–108
(DOI:10.1159/000208791)

A Novel Diagnostic Tool for Detecting Neonatal Infections Using Multiplex Polymerase Chain Reaction

Enomoto M. · Morioka I. · Morisawa T. · Yokoyama N. · Matsuo M.
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: May 08, 2008
Accepted: July 31, 2008
Published online: March 12, 2009
Issue release date: August 2009

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 3

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO

Abstract

Background: In newborns with infections, it is necessary to detect various pathogens rapidly and accurately, because the infections are often fatal when diagnosis is delayed. However, no diagnostic tools that rapidly detect pathogens causing neonatal infectious diseases are available. Objectives: To establish a rapid diagnostic tool using multiplex polymerase chain reaction (PCR) to detect 8 major pathogens that often cause neonatal infections, including Group B Streptococcus, Escherichia coli, Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, Ureaplasma urealyticum, herpes simplex virus, Cytomegalovirus, and Candida albicans, and to validate this tool in the neonatal intensive care unit (NICU). Methods: One hundred and thirty clinical samples were obtained from newborns with any infectious signs or histories. DNA was extracted from these samples and multiplex PCR was performed with a mixture of 8 primer pairs, all designed to amplify pathogenic DNA and produce different sizes of amplicons. Seventy-seven samples with suspicion of bacterial infections were also examined by bacterial culture to evaluate the accuracy of the multiplex PCR results. Results: Six of the 8 pathogens could be rapidly detected by our multiplex PCR method, within 3.5–4.5 h. These positive results led us to immediately diagnose and select proper drugs against each pathogen. In comparison with culture results, our test characteristics were as follows: specificity: 93%, negative predictive value: 96%, and concordance rate: 90%. Conclusions: We have established and validated a rapid diagnostic tool for detecting pathogens using multiplex PCR, which may be useful for the confirmed diagnosis of neonatal infections in the NICU.

© 2009 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: May 08, 2008
Accepted: July 31, 2008
Published online: March 12, 2009
Issue release date: August 2009

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 3

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.