Local Peritonectomy Highly Attracts Free Floating Intraperitoneal Colorectal Tumour Cells in a Rat ModelKönigsrainer I. · Zieker D. · Beckert S. · von Weyhern C. · Löb S. · Falch C. · Brücher B.L. · Königsrainer A. · Glatzle J.
1Department of General-, Visceral- and Transplant Surgery,2Department of Pathology, University of Tübingen,*equally contributing authors
Background/Aims: Intraperitoneal free cancer cells are associated with a higher risk of recurrence and a poorer prognosis in colorectal surgery. Tumour recurrence may occur early after surgery. One potential mechanism is the ability of peritoneal lesions to attract tumour cells. Methods: In Wag-Rija rats, the parietal peritoneum was resected on a defined area, a corresponding control area was marked in the same rat and colorectal tumour cells (CC531) were applied into the abdomen after surgery. Tissue was harvested 6 or 9 days after surgery to evaluate intra-abdominal tumour growth. Additionally tumour cells were applied 2 weeks after peritoneal resection to investigate tumour growth in a healed area of peritonectomy. Specimens were evaluated for macroscopic tumour spread, weight of the abdominal wall and maximal tumour thickness. Results: Macroscopic tumour spread, weight of the abdominal wall and maximal tumour thickness were significantly increased within the area of peritonectomy after both 6 and 9 days compared to the control area. However, only macroscopic tumour expansion was significantly increased in the healed area of peritonectomy. Conclusion: Peritoneal defects may play an important role in the pathogenesis of tumour implantation and might have some impact on tumour recurrence. The peritoneal damage should be kept as low as possible.
Department of Surgery, University of Tübingen
Hoppe-Seyler-Strasse 3, 72076 Tübingen (Germany)
Accepted: February 23, 2009
Published online: May 06, 2009
Number of Print Pages : 8
Cellular Physiology and Biochemistry (International Journal of Experimental Cellular Physiology, Biochemistry andPharmacology)
Vol. 23, No. 4-6, Year 2009 (Cover Date: 2009)
Journal Editor: F. Lang, Tübingen
ISSN: 1015–8987 (Print), eISSN: 1421–9778 (Online)
For additional information: http://www.karger.com/journals/cpb