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Gene Therapy of Cochlear Deafness

Present Concepts and Future Aspects

Editor(s): Ryan A.F. (La Jolla, Calif.) 
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Ryan AF (ed): Gene Therapy of Cochlear Deafness. Adv Otorhinolaryngol. Basel, Karger, 2009, vol 66, pp 87–98
(DOI:10.1159/000218209)
Paper

Adenoviral and AAV-Mediated Gene Transfer to the Inner Ear: Role of Serotype, Promoter, and Viral Load on In Vivo and In Vitro Infection Efficiencies

Luebke A.E.a · Rova C.b · Von Doersten P.G.b · Poulsen D.J.b
aDepartments of Neurobiology and Anatomy and Biomedical Engineering, University of Rochester Medical Center, Rochester, N.Y., and bDepartment of Biomedical and Pharmaceutical Sciences, COBRE Center for Structural and Functional Neuroscience, University of Montana, Missoula, Mont., USA Ryan AF (ed): Gene Therapy of Cochlear Deafness. Adv Otorhinolaryngol. Basel, Karger, 2009, vol 66, pp 87–98 (DOI:10.1159/000218209)

Abstract

The lack of effective treatments for many forms of hearing and vestibular disorders has produced interest in virally mediated gene therapies. However, to develop a gene therapy strategy that would successfully treat inner ear disorders, appropriate viral vectors capable of transfecting cochlear and support cells must be identified. While virally mediated gene transfer into the inner ear has been accomplished using herpes simplex type I virus, vaccinia virus, retroviruses, adenovirus, and adeno-associated virus (AAV), we will restrict our discussion to AAV and adenoviral vectors. Issues such as vector toxicity and load, viral serotype and backbone, and promoter specificity are discussed and contrasted for both in vivo vs. in vitro inner ear gene transfer.

 goto top of outline Author Contacts

Anne E. Luebke, PhD, Department of Biomedical Engineering, University of Rochester, Medical Center Box 603, 601 Elmwood Ave. MC68547, Rochester, NY 14642 (USA), Tel. +1 585 273 1635, Fax +1 585 276 5334, E-Mail Anne_Luebke@urmc.rochester.edu


 goto top of outline Article Information

Published online: June 02, 2009
Number of Print Pages : 12


 goto top of outline Publication Details

Book Serie: Advances in Oto-Rhino-Laryngology, Vol. 66, Year 2009

Editor(s): Arnold, W. (München)
ISSN: 0065-3071 (Print), eISSN: 1662-2847 (Online)

For additional information:
http://content.karger.com/ProdukteDB/produkte.asp?issn=0065-3071

Book Title: Gene Therapy of Cochlear Deafness (Present Concepts and Future Aspects)

Editor(s): Ryan AF (ed)

For additional information:
http://content.karger.com/ProdukteDB/produkte.asp?issn=0065-3071&volume=66


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