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Vol. 43, No. 4, 2009
Issue release date: July 2009
Section title: Original Paper
Caries Res 2009;43:308–313
(DOI:10.1159/000222659)

The Isolation of Bifidobacteria from Occlusal Carious Lesions in Children and Adults

Mantzourani M.a · Gilbert S.C.b · Sulong H.N.H.a · Sheehy E.C.b · Tank S.a · Fenlon M.a · Beighton D.a, b
aDental Institute, King’s College London, and bBiomedical Research Centre, Guy’s and St. Thomas’ Foundation Trust, London, UK

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 1/7/2009
Accepted: 4/6/2009
Published online: 6/3/2009
Issue release date: July 2009

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 1

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE

Abstract

The aim of this study was to enumerate and identify bifidobacteria from occlusal carious lesions in permanent and deciduous teeth. Samples of infected dentine were obtained from 24 active occlusal lesions in deciduous teeth and from 15 occlusal lesions in permanent teeth. Plaque samples from sound occlusal surfaces of 12 caries-free adults and 12 children were also obtained. The bifidobacterial strains were isolated in mupirocin-containing selective media, Gram-stained and subcultured for identification. Total bacterial counts were determined using fastidious anaerobic agar, and isolates were identified using genus-specific PCR primers and were confirmed by 16S rRNA sequencing. Bifidobacteria were isolated from 13 of the 15 occlusal lesions in the adults and formed 5.09 ± 2.11% of the total cultivable flora. In the children, bifidobacteria were isolated from 16 of the 24 occlusal lesions and formed 7.4 ± 2.6% of the total flora. No bifidobacteria were isolated from the occlusal surfaces of caries-free adults or children. A total of 424 bifidobacteria were identified and these were Bifidobacteriumdentium, Parascardovia denticolens, Scardoviainopicata, Bifidobacterium longum,Scardovia genomosp. C1 and Bifidobacterium breve. B. dentium was present in 14 out of the 16 bifidobacteria-positive samples from the lesions on the deciduous teeth and in 7 out of the 13 positive lesions in adults (p = 0.04). The present data suggest that bifidobacteria may play a role in the progression of occlusal caries lesions in both children and adults.

© 2009 S. Karger AG, Basel


  

Author Contacts

David Beighton
Department of Microbiology, Henry Wellcome Laboratories for Microbiology and Salivary Research, Dental Institute, King’s College London
Floor 17, Guys Tower, London Bridge, London SE1 9RT (UK)
Tel. +44 207 188 7465, Fax +44 207 188 7466, E-Mail david.beighton@kcl.ac.uk

  

Article Information

Received: January 7, 2009
Accepted after revision: April 6, 2009
Published online: June 3, 2009
Number of Print Pages : 6
Number of Figures : 1, Number of Tables : 1, Number of References : 27

  

Publication Details

Caries Research

Vol. 43, No. 4, Year 2009 (Cover Date: July 2009)

Journal Editor: Shellis R.P. (Bristol)
ISSN: 0008-6568 (Print), eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 1/7/2009
Accepted: 4/6/2009
Published online: 6/3/2009
Issue release date: July 2009

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 1

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

For additional information: http://www.karger.com/CRE


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