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Table of Contents
Vol. 84, No. 2, 2009
Issue release date: August 2009
Section title: Short Communication
Pharmacology 2009;84:68–73
(DOI:10.1159/000226123)

Genistein in the Presence of 17β-Estradiol Inhibits Proliferation of ERβ Breast Cancer Cells

Rajah T.T. · Du N. · Drews N. · Cohn R.
Department of Biological Sciences, DePaul University, Chicago, Ill., USA

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Article / Publication Details

First-Page Preview
Abstract of Short Communication

Received: 2/2/2009
Accepted: 4/15/2009
Published online: 6/26/2009

Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 1

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA

Abstract

Background/Aim:Genistein, a soy component, has been shown to have a biphasic proliferative effect in breast cancer cells, inhibiting in vitro cell proliferation at high concentrations (>10 μmol/l), while stimulating cell proliferation at lower concentrations (<10 μmol/l). However, epidemiological studies have shown an inverse correlation between the intake of genistein and the incidence of breast cancer. One of the possible reasons for this discrepancy could be the differing status of the estrogen receptor (ERα and/or ERβ). Genistein selectively binds to ERβ with strong affinity and thereby could be a potential chemotherapeutic agent against breast cancer of the ERα-negative and ERβ-positive type. Therefore, the objective of the present study was to determine whether the proliferative effects of genistein were caused by its activity as a selective ERβ agonist or merely as an antiestrogen. Method: This study was carried out in MDA-MB-231 (ERβ) and T47D (ERα and ERβ) human breast cancer cells. Cell proliferation was determined by the MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) assay. The cells were grown in estrogen-starved media and exposed to genistein at different concentrations for 72 h, either in the presence or absence of 17β-estradiol. Results: A significant decrease in cell proliferation was seen in MDA-MB-231 cells at low concentrations of genistein in the presence of 17β-estradiol, as compared to genistein alone. In T47D cells, which are known to have a predominance of ERα over ERβ, genistein showed a biphasic cell proliferative response both in the presence and absence of 17β-estradiol. Conclusions:Our results suggest that in cells with a predominance of ERα, genistein acts as an agonist to ERα, and in cells with ERβ alone, genistein most likely acts as an antiestrogen. Our results also suggest that genistein could be useful as a chemotherapeutic agent in premenopausal women with breast cancer of the ERα-negative and ERβ-positive type.


  

Author Contacts

Talitha T. Rajah, PhD
Department of Biological Sciences
DePaul University
2325 N. Clifton Ave, Chicago, IL 60614 (USA)
Tel. +1 773 325 8006, Fax +1 773 325 7596, E-Mail trajah@depaul.edu

  

Article Information

Received: February 2, 2009
Accepted after revision: April 15, 2009
Published online: June 26, 2009
Number of Print Pages : 6
Number of Figures : 2, Number of Tables : 1, Number of References : 25

  

Publication Details

Pharmacology (International Journal of Experimental and Clinical Pharmacology)

Vol. 84, No. 2, Year 2009 (Cover Date: August 2009)

Journal Editor: Donnerer J. (Graz), Billingsley M.L. (Hershey, Pa.), Maeyama K. (Matsuyama)
ISSN: 0031-7012 (Print), eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA


Article / Publication Details

First-Page Preview
Abstract of Short Communication

Received: 2/2/2009
Accepted: 4/15/2009
Published online: 6/26/2009

Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 1

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA


Copyright / Drug Dosage

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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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