Journal Mobile Options
Table of Contents
Vol. 91, No. 3, 1990
Issue release date: 1990
Add to my selection Citation Download
Int Arch Allergy Immunol 1990;91:224–231
(DOI:10.1159/000235121)
Original Paper

Western Blot Analysis of Water-Soluble Wheat Flour (Triticum vulgaris) Allergens

Pfeil T.a · Schwabl U.b · Ulmer W.T.b · König W.a
aInstitut für Medizinische Mikrobiologie und Immunologie, Arbeitsgruppe für Infektabwehrmechanismen, und bMedizinische Universitätsklinik «Bergmannsheil», Ruhr-Universität Bochum, FRG Int Arch Allergy Immunol 1990;91:224–231 (DOI:10.1159/000235121)

Abstract

Water-soluble wheat flour allergens were analyzed using the western blot technique. The sera of 130 bakers (42 with wheat flour allergy, 88 without allergy) were analyzed for IgE, IgG, and IgG4 binding towards wheat flour allergens. Three major allergens with molecular weights of 47, 17, and 15 kD were identified (Tri v Bd 47, Tri v Bd 17, and Tri v Bd 15; IUIS allergen nomenclature) by their ability to bind IgE from approximately 50% of the allergic sera. The specificity of the IgG was mostly directed against three high molecular weight components (134, 122, and 98 kD). In contrast, IgE and IgG4 was found frequently against the major allergens. 40% of healthy donors versus 12% of the asthmatic bakers had no detectable IgG4 against wheat flour extract. The biological activity of the water-soluble wheat flour components has been shown by their ability to induce histamine release from basophil leukocytes obtained from patients with bakers’ asthma. The release of histamine from basophil leukocytes significantly correlated with the presence of IgE antibodies against wheat flour components of 47, 17 and 15 kD. A regulatory role of wheat flour specific IgG4 in allergic diseases could not be evaluated by western blot analysis.

 goto top of outline Author Contacts

Correspondence to: Dr. W. König, Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Arbeitsgruppe Infektabwehrmechanismen, Ruhr-Universität Bochum, Universitätsstrasse 150, D–4630 Bochum (FRG)


 goto top of outline Article Information

Received: May 16, 1989
Accepted after revision: October 23, 1989
Published online: August 06, 2009
Number of Print Pages : 8


 goto top of outline Publication Details

International Archives of Allergy and Immunology

Vol. 91, No. 3, Year 1990 (Cover Date: 1990)

Journal Editor: Valenta R. (Vienna)
ISSN: 1018-2438 (Print), eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.