Faecally Derived Hydrolytic Enzymes from Dermatophagoides pteronyssinus: Physicochemical Characterisation of Potential AllergensStewart G.A. · Lake F.R. · Thompson P.J.
aWestern Australian Research Institute for Child Health, Princess Margaret Hospital, and bDepartment of Medicine, University of Western Australia, Perth, Australia
The previous findings that the group I and III mite allergens, and amylase present in mite faeces are hydrolytic enzymes has prompted a study to determine whether this material contains other enzymes which could be allergenic. Thus, spent growth medium devoid of whole Dermatophagoides pteronyssinus mites was shown to contain glucoamylase, lipase and lysozyme in addition to the cysteine protease, serine protease and amylase activities associated with the above allergens, respectively. All of these enzymes are probably associated with mite digestive processes. They were rapidly solubilised, heterogeneous with regard to charge (pI in the range 4–8) and demonstrated maximum biochemical activity in the neutral pH range. Three serine proteases were detected and comprised a chymotrypsin-like, a trypsin-like and an unclassified enzyme with pI of 4.1 and 5.3, 8.5 and 7.1, respectively. Only one cysteine protease was observed, which paralleled immunochemically identified Der p I in a variety of assays. It was shown to cleave at lysyl residues and could be inhibited by the specific cysteine protease inhibitor, E-64. The remaining serine proteases, glucoamylase, lipase and lysozyme represent potential allergens.
Correspondence to: Dr. G.A. Stewart, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Thomas Street, Subiaco, Perth, WA 6008 (Australia)
Received: January 29, 1991
Accepted: March 18, 1991
Published online: August 07, 2009
Number of Print Pages : 9
International Archives of Allergy and Immunology
Vol. 95, No. 2-3, Year 1991 (Cover Date: 1991)
Journal Editor: Valenta R. (Vienna)
ISSN: 1018-2438 (Print), eISSN: 1423-0097 (Online)
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